Publication Details

Category Text Publication
Reference Category Book chapters
DOI 10.1002/9780470054581.eib320
Title (Primary) Flow cytometry
Title (Secondary) Encyclopedia of industrial biotechnology: bioprocess, bioseparation, and cell technology, Vol. 4
Author Müller, S.; Bley, T.
Publisher Flickinger, M.C.
Year 2010
Department UMB
Page From 2451
Page To 2467
Language englisch
Keywords flow cytometry; single cell analysis; population dynamics; community structure; biotechnological process optimization
Abstract Microorganisms are widely used in biotechnology and present a wide biodiversity including yeast, algae, protozoa and bacteria. Because of their high abundances, and their large variety of metabolic activities which makes them ubiquitous, microorganisms represent a key component in environmental and biotechnical studies and applications. To enhance our understanding of the heterogeneity and complexity of the microbial consortia, both in term of composition and functioning, their direct analysis must be done on a single cell level and requires a non-invasive interrogation of the cells. The developments of analytical flow and static cytometry, together with advances in the field of fluorescent probes have enabled the measurement of a wide range of cell characteristics. The study of technical systems where microbial cells interact within an artificially engineered environment (e.g. fermentation, bio-remediation, bio-transformation) is important for the biotechnical industry. Accurate measurements relating to cell proliferation, viability and scatter properties of cells are essential if informed decisions about a process are to be made. In this way, products can be harvested at optimal concentrations and inducible systems can be activated at the appropriate time so that high product yields are achieved. In the review are highlighted newly developed techniques and recent advances in this field.
Persistent UFZ Identifier
Müller, S., Bley, T. (2010):
Flow cytometry
In: Flickinger, M.C. (ed.)
Encyclopedia of industrial biotechnology: bioprocess, bioseparation, and cell technology, Vol. 4
Wiley, Oxford, p. 2451 - 2467