Publication Details

Category Text Publication
Reference Category Journals
DOI 10.3109/17435391003605455
Title (Primary) Physical-chemical characterization of tungsten carbide nanoparticles as a basis for toxicological investigations
Author Meissner, T.; Kühnel, D.; Busch, W. ORCID logo ; Oswald, S.; Richter, V.; Michaelis, A.; Schirmer, K.; Potthoff, A.
Source Titel Nanotoxicology
Year 2010
Department BIOTOX
Volume 4
Issue 2
Page From 196
Page To 206
Language englisch
Keywords Particle characterization; nanoparticles; suspension preparation; tungsten carbide; toxicity
Abstract One task in risk assessment of engineered nanoparticles is toxicological studies. A suitable interpretation of these investigations demands a comprehensive physical-chemical characterization. Here, we present an approach to gain well-dispersed nanoparticles in physiological media. Therefore, a step-by-step procedure is demonstrated on two different tungsten carbide nanopowders which can be transferred to other powders. The procedure includes a comprehensive powder characterization, followed by a preparation of a non-physiologic, electrostatically stable nanoparticle suspension and finally closes with investigations of the particles' behavior in different physiological media. Our study showed that the particles agglomerate in protein-free media. In this context, dependencies of mass- and surface-based nanoparticle concentrations as well as of different physiological media were analyzed. In the presence of bovine serum albumin (BSA) or serum, the agglomeration process is decelerated or, at the appropriate protein amount, prevented.
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10294
Meissner, T., Kühnel, D., Busch, W., Oswald, S., Richter, V., Michaelis, A., Schirmer, K., Potthoff, A. (2010):
Physical-chemical characterization of tungsten carbide nanoparticles as a basis for toxicological investigations
Nanotoxicology 4 (2), 196 - 206 10.3109/17435391003605455