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Title (Primary) Natural stable isotope fractionation for the assessment of hydrocarbon degradation
Title (Secondary) Experimental protocols and appendices
Author Meckenstock, R.U.; Richnow, H.H.
Publisher Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V.
Journal Handbook of Hydrocarbon and Lipid Microbiology
Year 2010
Department ISOBIO
Volume Vol. 5, Part 2
Page From 3604
Page To 3611
Language englisch
UFZ inventory Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han
Abstract In recent years, the use of compound specific isotope analysis (CSIA) to measure stable isotope fractionation developed into one of the most important tools for assessing biodegradation of aromatic hydrocarbons in contaminated groundwater. Biodegradation reactions are often accompanied by shifts in the stable isotope ratios of the naturally abundances of e.g., 13C/12C, 2H/1H, or other elements of the compound of interest. This isotope fractionation is measured in the residual substrate fraction sampled from monitoring wells by means of CSIA. If laboratory degradation experiments have been performed to obtain stable isotope fractionation factors for a given compound and reaction one can use the Rayleigh equation to calculate the extent of biodegradation in the environment. The big advantage of the stable isotope fractionation approach is that one can even quantify the extent of biodegradation in extremely complex matrices such as aquifers. Several field studies successfully showed astonishing accuracy of the method to determine biodegradation rates on contaminated field sites. Nowadays, stable isotope tools are widely accepted by authorities as a reliable tool to prove biodegradation in natural attenuation. Here, we give a brief introduction how to use CSIA for assessing stable isotope fractionation of aromatic hydrocarbons together with a few selected successful examples of application.
Persistent UFZ Identifier
Meckenstock, R.U., Richnow, H.H. (2010):
Natural stable isotope fractionation for the assessment of hydrocarbon degradation
In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.)
Experimental protocols and appendices
Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 2
Springer International Publishing, Cham, p. 3604 - 3611