Publication Details |
| Category | Text Publication |
| Reference Category | Journals |
| DOI | 10.1016/j.chemosphere.2009.10.009 |
| Title (Primary) | White-rot fungus-mediated degradation of the analgesic ketoprofen and identification of intermediates by HPLC-DAD-MS and NMR |
| Author | Marco-Urrea, E.; Pérez-Trujillo, M.; Cruz-Morató, C.; Caminal, G.; Vicent, T. |
| Source Titel | Chemosphere |
| Year | 2010 |
| Department | ISOBIO |
| Volume | 78 |
| Issue | 4 |
| Page From | 474 |
| Page To | 481 |
| Language | englisch |
| Keywords | Ketoprofen; Biodegradation; Trametes versicolor; HPLC-DAD-MS; NMR |
| Abstract | Ketoprofen is a nonsteroidal anti-inflammatory drug that has been detected in the environment in the range of ng L-1-µg L-1 due to its low degradability in some wastewater treatment plants. In this study, the use of the white-rot fungus Trametes versicolor to effectively degrade ketoprofen in a defined liquid medium was assessed. The fungus eliminated ketoprofen to nondetectable levels in 24 h when it was added at 10 mg L-1 whereas at low concentration of 40 µg L-1 it was almost completely removed (95%) after 5 h. Low extracellular laccase activity was detected in the T. versicolor cultures but the addition of the laccase-mediator system did not lead to ketoprofen oxidation. The cytochrome P-450 inhibitor 1-aminobenzotriazole reduced ketoprofen oxidation. These data suggest that the first oxidation step is cytochrome P450 mediated. During time-course degradation experiments, three intermediates were structurally elucidated and quantified by HPLC-DAD-MS and NMR: 2-[3-(4-hydroxybenzoyl)phenyl]-propanoic acid, 2-[(3-hydroxy(phenyl)methyl)phenyl]-propanoic acid, and 2-(3-benzoyl-4-hydroxyphenyl)-propanoic acid. The latter was reported for the first time in biological systems. After 7 d of incubation, only small amounts of 2-[(3-hydroxy(phenyl)methyl)phenyl]-propanoic acid (0.08 mg) remained in the liquid medium in comparison with the initial ketoprofen dose (1.0 mg), suggesting possible mineralization of ketoprofen. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10272 |
| Marco-Urrea, E., Pérez-Trujillo, M., Cruz-Morató, C., Caminal, G., Vicent, T. (2010): White-rot fungus-mediated degradation of the analgesic ketoprofen and identification of intermediates by HPLC-DAD-MS and NMR Chemosphere 78 (4), 474 - 481 10.1016/j.chemosphere.2009.10.009 |
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