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Title (Primary) Biodegradation of the analgesic naproxen by Trametes versicolor and identification of intermediates using HPLC-DAD-MS and NMR
Author Marco-Urrea, E.; Pérez-Trujillo, M.; Blánquez, P.; Vicent, T.; Caminal, G.;
Journal Bioresource Technology
Year 2010
Department ISOBIO;
Volume 101
Issue 7
Language englisch;
Keywords Naproxen; Trametes versicolor; NMR; Cytochrome P450; Laccase
Abstract The white-rot fungus Trametes vesicolor degraded naproxen (10 mg L-1) in a liquid medium to non-detectable levels after 6 h. When naproxen was added in the range of concentrations typically found in the environment (55 µg L-1), it was almost completely degraded (95%) after 5 h. In vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. A noticeable inhibition on naproxen degradation was also observed when the cytochrome P450 inhibitor 1-aminobenzotriazole was added to the fungal cultures. These data suggest that both enzymatic systems could play a role in naproxen degradation. 2-(6-hydroxynaphthalen-2-yl)propanoic acid and 1-(6-methoxynaphthalen-2-yl)ethanone were structurally elucidated by HPLC-DAD-MS and NMR as degradation intermediates of naproxen. After 6 h of incubation, both parent compound and intermediates disappeared from the medium. The non-toxicity of the treated medium was confirmed by Microtox test.
ID 10271
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10271
Marco-Urrea, E., Pérez-Trujillo, M., Blánquez, P., Vicent, T., Caminal, G. (2010):
Biodegradation of the analgesic naproxen by Trametes versicolor and identification of intermediates using HPLC-DAD-MS and NMR
Bioresour. Technol. 101 (7), 2159 - 2166