Publication Details |
Category | Text Publication |
Reference Category | Journals |
DOI | 10.1016/j.biortech.2009.11.019 |
Title (Primary) | Biodegradation of the analgesic naproxen by Trametes versicolor and identification of intermediates using HPLC-DAD-MS and NMR |
Author | Marco-Urrea, E.; Pérez-Trujillo, M.; Blánquez, P.; Vicent, T.; Caminal, G. |
Source Titel | Bioresource Technology |
Year | 2010 |
Department | ISOBIO |
Volume | 101 |
Issue | 7 |
Page From | 2159 |
Page To | 2166 |
Language | englisch |
Keywords | Naproxen; Trametes versicolor; NMR; Cytochrome P450; Laccase |
Abstract | The white-rot fungus Trametes vesicolor degraded naproxen (10 mg L-1) in a liquid medium to non-detectable levels after 6 h. When naproxen was added in the range of concentrations typically found in the environment (55 µg L-1), it was almost completely degraded (95%) after 5 h. In vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. A noticeable inhibition on naproxen degradation was also observed when the cytochrome P450 inhibitor 1-aminobenzotriazole was added to the fungal cultures. These data suggest that both enzymatic systems could play a role in naproxen degradation. 2-(6-hydroxynaphthalen-2-yl)propanoic acid and 1-(6-methoxynaphthalen-2-yl)ethanone were structurally elucidated by HPLC-DAD-MS and NMR as degradation intermediates of naproxen. After 6 h of incubation, both parent compound and intermediates disappeared from the medium. The non-toxicity of the treated medium was confirmed by Microtox test. |
Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10271 |
Marco-Urrea, E., Pérez-Trujillo, M., Blánquez, P., Vicent, T., Caminal, G. (2010): Biodegradation of the analgesic naproxen by Trametes versicolor and identification of intermediates using HPLC-DAD-MS and NMR Bioresour. Technol. 101 (7), 2159 - 2166 10.1016/j.biortech.2009.11.019 |