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Title (Primary) Potassium deficiency results in accumulation of ultra-high molecular weight poly-β-hydroxybutyrate in a methane-utilizing mixed culture
Author Helm, J.; Wendlandt, K.-D.; Jechorek, M.; Stottmeister, U.;
Journal Journal of Applied Microbiology
Year 2008
Department UBT;
Volume 105
Issue 4
Language englisch;
Keywords methanotrophic bacteria; Methylocystis sp. GB25; poly-ß-hydroxybutyrate; potassium deficiency; ultra-high molecular weight PHB
Abstract Aims: To investigate the effect of various single nutrient deficiencies on poly-ß-hydroxybutyrate (PHB) biosynthesis in a methane-utilizing mixed culture (dominant species Methylocystis sp. GB 25 DSM 7674).Methods and Results: Poly-ß-hydroxybutyrate accumulation experiments were performed in 7 and 70 l bioreactors and initiated by potassium, sulfur or iron deficiency. After 24 h the PHB content reached levels of 33·6%, 32·6% and 10·4% respectively. Interestingly a polymer with an ultra-high average-weight molecular weight (Mw) of 3·1 MDa was accumulated under potassium-limited conditions. When sulfur and iron were lacking Mw were lower by 20·6 and 41·6%. Potassium-deficiency experiments were furthermore characterized by a maximum specific PHB formation rate 0·08 g g-1residual biomass (R) h-1 and a yield coefficient of 0·45 g PHB g-1 CH4.Conclusions: Biosynthesis of an ultra-high Mw PHB in a methane-utilizing mixed culture can be induced by potassium deficiency.Significance and Impact of the Study: This study greatly extends the knowledge in the field of bacterial biopolymer formation with gaseous substrates. The special system used here combines the use of methane a low-cost substrate available from natural and renewable sources with the possibility of employing a mixed-culture in an open system for the synthesis of a high-value product.
ID 1018
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=1018
Helm, J., Wendlandt, K.-D., Jechorek, M., Stottmeister, U. (2008):
Potassium deficiency results in accumulation of ultra-high molecular weight poly-β-hydroxybutyrate in a methane-utilizing mixed culture
J. Appl. Microbiol. 105 (4), 1054 - 1061