Publication Details

Reference Category Book chapters
DOI / URL link
Title (Primary) In situ microcosms studies to characterize microbial processes in the field
Title (Secondary) Experimental protocols and appendices
Author Kästner, M.; Richnow, H.H.;
Publisher Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V.;
Journal Handbook of Hydrocarbon and Lipid Microbiology
Year 2010
Department ISOBIO; UBT;
Volume Vol. 5, Part 1
Language englisch;
UFZ inventory Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han
Abstract A concept of in situ microcosms, which allows analyzing structure, function, and activity of microbial communities in situ, as well as the enrichment of microorganisms for subsequent cultivation in laboratory experiments is described. The concept is based on the application of stable isotope-labeled compounds supplied within the microcosm system. Microorganisms capable of utilizing these substrates as carbon or nitrogen sources become labeled upon metabolism and biosynthesis. Stable isotope probing (SIP) of biomarker molecules such as?fatty acids, proteins, or nucleic acids allows tracing the flux of the label into the biomass and?offers potential for linking structure and function of the active microbial communities.
ID 10146
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10146
Kästner, M., Richnow, H.H. (2010):
In situ microcosms studies to characterize microbial processes in the field
In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.)
Experimental protocols and appendices
Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 1
Springer International Publishing, Cham, p. 3504 - 3511