Publication Details |
| Category | Text Publication |
| Reference Category | Book chapters |
| DOI | 10.10073/978-3-540-77587-4_273 |
| Title (Primary) | In situ microcosms studies to characterize microbial processes in the field |
| Title (Secondary) | Experimental protocols and appendices |
| Author | Kästner, M.; Richnow, H.H. |
| Publisher | Timmis, K.N.; McGenity, T.; van den Meer, J.R.; de Lorenzo, V. |
| Source Titel | Handbook of Hydrocarbon and Lipid Microbiology |
| Year | 2010 |
| Department | ISOBIO; UBT |
| Volume | Vol. 5, Part 1 |
| Page From | 3504 |
| Page To | 3511 |
| Language | englisch |
| UFZ inventory | Leipzig, Bibliothek, Hauptlesesaal, 00358415, 09-1344 DK: 579.222.5 Han |
| Abstract | A concept of in situ microcosms, which allows analyzing structure, function, and activity of microbial communities in situ, as well as the enrichment of microorganisms for subsequent cultivation in laboratory experiments is described. The concept is based on the application of stable isotope-labeled compounds supplied within the microcosm system. Microorganisms capable of utilizing these substrates as carbon or nitrogen sources become labeled upon metabolism and biosynthesis. Stable isotope probing (SIP) of biomarker molecules such as?fatty acids, proteins, or nucleic acids allows tracing the flux of the label into the biomass and?offers potential for linking structure and function of the active microbial communities. |
| Persistent UFZ Identifier | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10146 |
| Kästner, M., Richnow, H.H. (2010): In situ microcosms studies to characterize microbial processes in the field In: Timmis, K.N., McGenity, T., van den Meer, J.R., de Lorenzo, V. (eds.) Experimental protocols and appendices Handbook of Hydrocarbon and Lipid Microbiology Vol. 5, Part 1 Springer International Publishing, Cham, p. 3504 - 3511 10.10073/978-3-540-77587-4_273 |
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