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Title (Primary) Proteomic signatures of the zebrafish (Danio rerio) embryo: sensitivity and specificity in toxicity assessment of chemicals
Author Hanisch, K.; Küster, E.; Altenburger, R.; Gündel, U.;
Journal International Journal of Proteomics
Year 2010
Department BIOTOX;
Volume 2010
Language englisch;
Abstract Studies using embryos of the zebrafish Danio rerio (DarT) instead of adult fish for characterising the (eco-) toxic potential of chemicals have been proposed as animal replacing methods. Effect analysis at the molecular level might enhance sensitivity, specificity, and predictive value of the embryonal studies. The present paper aimed to test the potential of toxicoproteomics with zebrafish eleutheroembryos for sensitive and specific toxicity assessment. 2-DE-based toxicoproteomics was performed applying low-dose (EC10) exposure for 48?h with three-model substances Rotenone, 4,6-dinitro-o-cresol (DNOC) and Diclofenac. By multivariate "pattern-only" PCA and univariate statistical analyses, alterations in the embryonal proteome were detectable in nonetheless visibly intact organisms and treatment with the three substances was distinguishable at the molecular level. Toxicoproteomics enabled the enhancement of sensitivity and specificity of the embryonal toxicity assay and bear the potency to identify protein markers serving as general stress markers and early diagnosis of toxic stress.
ID 10038
Persistent UFZ Identifier https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=10038
Hanisch, K., Küster, E., Altenburger, R., Gündel, U. (2010):
Proteomic signatures of the zebrafish (Danio rerio) embryo: sensitivity and specificity in toxicity assessment of chemicals
International Journal of Proteomics 2010 , Article ID 630134 - 13