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Referenztyp Zeitschriften
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Titel (primär) Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques
Autor Bombach, P.; Chatzinotas, A.; Neu, T.R.; Kästner, M.; Lueders, T.; Vogt, C.;
Journal / Serie FEMS Microbiology Ecology
Erscheinungsjahr 2010
Department ISOBIO; UMB; FLOEK; UBT;
Band/Volume 71
Heft 2
Sprache englisch;
Keywords Toluene; stable isotope probing (SIP); in situ microcosm; sulfate-reducing; biodegradation
Abstract A toluene-degrading microbial consortium was enriched directly in a BTEX-contaminated aquifer under sulfate-reducing conditions using in situ microcosms consisting of toluene-loaded activated carbon pellets. Degradation of toluene and concomitant sulfide production by the consortium was subsequently demonstrated in laboratory microcosms. The consortium was physiologically and phylogenetically characterized by isotope tracer experiments using nonlabeled toluene, [13C]-a-toluene or [13C7]-toluene as growth substrates. Cells incubated with [13C]-a-toluene or [13C7]-toluene incorporated 8-15 at.%13C and 51-57 at.%13C into total lipid fatty acids, respectively, indicating a lower specific incorporation of 13C from [13C7]-toluene. In order to identify the toluene-assimilating bacteria, the incorporation of carbon from both [13C]-a-toluene and [13C7]-toluene into rRNA was analyzed by stable isotope probing. Time and buoyant density-resolved 16S rRNA gene-based terminal restriction fragment length polymorphism profiles, combined with cloning and sequencing, revealed that an uncultured bacterium (99% sequence similarity) related to the genus Desulfocapsa was the main toluene-degrading organism in the consortium. The ratio of the respective terminal restriction fragments changed over time, indicating trophic interactions within this consortium.
ID 9815
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9815
Bombach, P., Chatzinotas, A., Neu, T.R., Kästner, M., Lueders, T., Vogt, C. (2010):
Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques
FEMS Microbiol. Ecol. 71 (2), 237 - 246