Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1046/j.1365-2958.1997.2961658.x
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Titel (primär) DnaA protein stimulates polA gene expression in Escherichia coli
Autor Quiñones, A.; Wandt, G.; Kleinsteuber, S. ORCID logo ; Messer, W.
Quelle Molecular Microbiology
Erscheinungsjahr 1997
Department UMB
Band/Volume 23
Heft 6
Seite von 1193
Seite bis 1202
Sprache englisch

The polA gene of Escherichia coli encodes DNA polymerase I that is involved in DNA replication and repair. Despite the wide knowledge about structure and function of DNA polymerase I, there is little insight into the regulatory mechanisms involved in polA expression. DnaA is the initiator protein for DNA replication in E. coli. There are two putative DnaA-binding sites within the extended promoter region of polA. In this work we studied the influence of altered levels of DnaA protein on polA expression. We found that DnaA overproduction increases polA expression in stationary-phase cultures. The stimulation effect was independent of rpoS, which encodes the sigma factor for stationary-phase-inducible genes. However, it was modulated by ppGpp. Comparative S1 analyses revealed that the induction was based on transcriptional stimulation. Footprint-ing experiments demonstrated that DnaA binds only to the proximal DnaA box near the polA promoter. These results suggest an additional role for DnaA as transcriptional activator of polA at least under certain physiological conditions.

dauerhafte UFZ-Verlinkung
Quiñones, A., Wandt, G., Kleinsteuber, S., Messer, W. (1997):
DnaA protein stimulates polA gene expression in Escherichia coli
Mol. Microbiol. 23 (6), 1193 - 1202 10.1046/j.1365-2958.1997.2961658.x