Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1111/j.1574-6941.1997.tb00428.x
Titel (primär) Characterisation of a manganese-reducing, toluene-degrading enrichment culture
Autor Langenhoff, A.; Nijenhuis, I.; Tan, N.C.G.; Briglia, M.; Zehnder, A.J.B.; Schraa, G.
Quelle FEMS Microbiology Ecology
Erscheinungsjahr 1997
Department ISOBIO; UBT
Band/Volume 24
Heft 2
Seite von 113
Seite bis 125
Sprache englisch
Abstract

A bacterial culture (LET-13) was enriched, which uses toluene as sole carbon and energy source, and manganese oxide as terminal electron acceptor. The culture is able to degrade a variety of substituted monoaromatic compounds like p-hydroxy-benzylalcohol, p-hydroxy-benzaldehyde, p-hydroxy-benzoate, phenol and the three isomers of cresol. Benzene, ethylbenzene, all xylenes and naphthalene were not degraded under the experimental conditions used. Based on the results of growth experiments and the detection of intermediates, it is concluded that toluene is degraded via a methyl hydroxylation. A possible side reaction can lead to the formation of cresol. The organisms in the culture look similar; motile rods, which are Gram-negative, oxidase-negative and catalase-negative. The culture was partly identified by phylogenetic analysis of cloned rDNA sequences. The phylogenetic analysis showed that at least two major groups of bacteria are present. One group of bacteria shows 70–80% similarity (based on 16S rRNA gene sequence data) with the Bacteroides-Cytophaga group, and one group consists of members of the β-subclass of the Proteobacteria.

dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9454
Langenhoff, A., Nijenhuis, I., Tan, N.C.G., Briglia, M., Zehnder, A.J.B., Schraa, G. (1997):
Characterisation of a manganese-reducing, toluene-degrading enrichment culture
FEMS Microbiol. Ecol. 24 (2), 113 - 125 10.1111/j.1574-6941.1997.tb00428.x