Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/S0304-3940(97)00332-7
Titel (primär) Conversion of biocytin labelled cells and structures for the confocal laser-scanning method
Autor Hilbig, H.; Müller, A.
Quelle Neuroscience Letters
Erscheinungsjahr 1997
Department EXPOEPID
Band/Volume 227
Heft 3
Seite von 209
Seite bis 211
Sprache englisch
Keywords Biocytin; Silver substitution; Brain
Abstract

The method for converting biocytin preparations of brain sections fills a gap in the application of confocal laser-scanning microscopy. Both neuronal and non-neuronal structures are converted. The background remains free of staining. The protocol can be applied to old and already existing biocytin-(diaminobenzidine)-nickel preparations which are then made accessible to evaluation with the laser-scanning microscope by the substitution of nickel with silver-gold. Sodium thiosulphate is used to remove the unbound silver. The reflection image of the laser-scanning microscopy provides more information than the transmission image.

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Hilbig, H., Müller, A. (1997):
Conversion of biocytin labelled cells and structures for the confocal laser-scanning method
Neurosci. Lett. 227 (3), 209 - 211 10.1016/S0304-3940(97)00332-7