Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1016/S0304-3940(97)00332-7 |
Titel (primär) | Conversion of biocytin labelled cells and structures for the confocal laser-scanning method |
Autor | Hilbig, H.; Müller, A. |
Journal / Serie | Neuroscience Letters |
Erscheinungsjahr | 1997 |
Department | EXPOEPID |
Band/Volume | 227 |
Heft | 3 |
Seite von | 209 |
Seite bis | 211 |
Sprache | englisch |
Keywords | Biocytin; Silver substitution; Brain |
Abstract | The method for converting biocytin preparations of brain sections fills a gap in the application of confocal laser-scanning microscopy. Both neuronal and non-neuronal structures are converted. The background remains free of staining. The protocol can be applied to old and already existing biocytin-(diaminobenzidine)-nickel preparations which are then made accessible to evaluation with the laser-scanning microscope by the substitution of nickel with silver-gold. Sodium thiosulphate is used to remove the unbound silver. The reflection image of the laser-scanning microscopy provides more information than the transmission image. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=9327 |
Hilbig, H., Müller, A. (1997): Conversion of biocytin labelled cells and structures for the confocal laser-scanning method Neurosci. Lett. 227 (3), 209 - 211 |