Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Sensor for amperometric determination of ammonia and ammonia forming enzyme reactions
Autor Strehlitz, B.; Gründig, B.; Kopinke, H.;
Journal / Serie Analytica Chimica Acta
Erscheinungsjahr 2000
Department UBZ; UBT_alt;
Band/Volume 403
Sprache englisch;
Keywords amperometric enzyme sensor; polyaniline film; ammonia; screen printed electrode; urea
Abstract

The amperometric ammonia determination by use of a polyaniline-modified screen printed Pt–C electrode at 0.3 V (versus Ag/AgCl on the strip) is shown. The electrochemical pulse polarization method is used for the modification. The effects of basic electrode material, applied potential, pH of buffer solution and possible interferences of other ions were studied. The polyaniline-modified Pt–C electrode measures (NH4)2SO4 with a detection limit of 5 μM, a linear range up to 1 mM and a sensitivity of 40 ± 20 μA cm−2 mM−1. A new generation of enzyme sensors arises from the immobilization of ammonia-forming enzymes in a poly(carbamoyl)sulphonate hydrogel matrix on the working electrode as it is shown for urease to detect urea.

ID 7693
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=7693
Strehlitz, B., Gründig, B., Kopinke, H. (2000):
Sensor for amperometric determination of ammonia and ammonia forming enzyme reactions
Anal. Chim. Acta 403 , 11 - 23