Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s10532-007-9156-0
Volltext Shareable Link
Titel (primär) Kinetics of chlorobenzene biodegradation under reduced oxygen levels
Autor Balcke, G.U.; Wegener, S.; Kiesel, B.; Benndorf, D.; Schlömann, M.; Vogt, C.
Journal / Serie Biodegradation
Erscheinungsjahr 2008
Band/Volume 19
Heft 4
Seite von 507
Seite bis 518
Sprache englisch
Keywords Hypoxic; Kinetics; Chlorobenzene; Optode; Chlorocatechol 1,2-dioxygenase; Oxygen affinity
Abstract Focussing on the role of chlorocatechol 1,2-dioxygenase (CC12O), an oxygen-dependent key enzyme in the aerobic catabolism of chlorobenzene (CB), Pseudomonas veronii strain UFZ B549, Acidovorax facilis strain UFZ B530, and a community of indigenous groundwater bacteria were amended with CB degradation under either oxic or hypoxic conditions. All cultures readily degraded CB at high oxygen availability, but had differing abilities to completely degrade CB when exposed to oxygen limitation. For the three cultures very distinct oxygen half-saturation constants (0.3-11.7 µM) for the respective CC12Os were obtained and protein analysis showed that high affinity-type A. facilis and low affinity-type P. veronii express CC12Os, which belong to different structural clusters. From this a functional relation between CC12O type and the ability to cope with efficient ring fission under oxygen limitation is anticipated. Extremely high oxygen affinities for CC12Os support the assumption that truly oxic environments are not an essential requirement to degrade chloro(aromatic) compounds. Tiny quantities of oxygen permanently re-supplied will sufficiently maintain the growth of microaerophilic specialists with the ability to transform chloro(aromatics) via catechol intermediates.
dauerhafte UFZ-Verlinkung
Balcke, G.U., Wegener, S., Kiesel, B., Benndorf, D., Schlömann, M., Vogt, C. (2008):
Kinetics of chlorobenzene biodegradation under reduced oxygen levels
Biodegradation 19 (4), 507 - 518