Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1016/S0168-1656(00)00408-9 |
Titel (primär) | Producing poly-3-hydroxybutyrate with a high molecular mass from methane |
Autor | Wendlandt, K.-D.; Jechorek, M.; Helm, J.; Stottmeister, U. |
Quelle | Journal of Biotechnology |
Erscheinungsjahr | 2001 |
Department | UBT; UBT_alt; SAN |
Band/Volume | 86 |
Heft | 2 |
Seite von | 127 |
Seite bis | 133 |
Sprache | englisch |
Keywords | Poly-3-hydroxybutyrate; Methanotrophs; Methane; Cultivation; Biosynthesis; High molecular mass; Methylocystis sp. GB 25 DSMZ 7674 |
Abstract | Poly-3-hydroxybutyrate (PHB) and other polyesters can be produced by various species of bacteria. Of the possible carbon sources, methane could provide a suitable substrate for the production of PHB. Methane is cheap and plentiful – not only as natural gas, but also as biogas. The methanotrophic strain Methylocystis sp. GB 25 DSMZ 7674 is able to accumulate PHB in a brief non-sterile process. The studies were carried out using a 7-l and a 70-l pressure bioreactor. Cultivation was performed in two stages: a continuous growth phase (dilution rate 0.17 h−1) and a PHB accumulation phase under deficiency conditions of an essential nutrient (ammonium, phosphorus or magnesium) in batch culture. The PHB content of biomass was as high as 51%; efficiency was highest during the first 5 h of the product formation process. The maximum PHB yield relative to the methane consumed was estimated to be 0.55 g g−1. The PHB produced is of very high quality, having a high molecular mass of up to 2.5×106 Da. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6927 |
Wendlandt, K.-D., Jechorek, M., Helm, J., Stottmeister, U. (2001): Producing poly-3-hydroxybutyrate with a high molecular mass from methane J. Biotechnol. 86 (2), 127 - 133 10.1016/S0168-1656(00)00408-9 |