Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1177/026119290102900321
Titel (primär) Metabolic activity in primary cultures of fish hepatocytes
Autor Segner, H.; Cravedi, J.
Journal / Serie ATLA-Alternatives to Laboratory Animals
Erscheinungsjahr 2001
Department OEC; COE
Band/Volume 29
Heft 3
Seite von 251
Seite bis 257
Sprache englisch
Keywords hepatocytes; teleost fish; biotransformation; in vitro culture; cytochrome P4501A
Abstract In aquatic toxicology, isolated liver cells from fish can be used as a tool to generate initial information on the hepatic metabolism of xenobiotics, and on the mechanisms of xenobiotic activation or deactivation. This isolation of teleost liver cells is achieved by enzymic dissociation, and monolayer cultures of fish hepatocytes in serum-free medium maintain good viability for 3-8 days. During in vitro culture, fish liver cells express stable levels of phase I and phase II enzymes, such as cytochrome P4501A or glutathione S-transferase, and the cells show an induction of biotransformation enzymes after exposure to xenobiotics. The xenobiotic metabolite pattern produced by fish hepatocytes in vitro is generally similar to that observed in vivo. Limitations to more-intensive application of cultured fish hepatocytes as a screen in aquatic hazard assessment are partly due to the rather limited scope of existing studies, i.e. the focus on one particular species (rainbow trout), and on one particular biotransformation enzyme (cytochrome P4501A), as well as a lack of comparative in vitro/in vivo studies.
dauerhafte UFZ-Verlinkung
Segner, H., Cravedi, J. (2001):
Metabolic activity in primary cultures of fish hepatocytes
ATLA-Altern. Lab. Anim. 29 (3), 251 - 257