Details zur Publikation
|DOI / URL||Link|
|Titel (primär)||Surface properties of bacteria from different wastewater treatment plants|
|Autor||Martienssen, M.; Reichel, O.; Kohlweyer, U.;|
|Journal / Serie||Acta Biotechnologica|
The surface properties of the individual members of degradative biocommunities isolated from different laboratory and natural populations were characterized. The bacterial strains isolated from a given origin and degrading a given substrate varied with respect to their hydrophobic and electrostatic properties (e.g. contact angle, adsorption to hexadecane, isoelectric point, adsorption of anionic orcationic dyes). However, despite their specific surface characteristics, in most cases the net charge properties of different bacterial strains (characterized by the zeta potential profiles of the bacteria in relation to the pH) were found to be related to the substrate the bacteria were able to degrade as well as to the consortium the bacteria were isolated from. For one group of specialized bacteria, only oneor at most two characteristic zeta potential profiles were measured.
Compared to the differences between different strains, the zeta potential profiles of individual strains were only slightly affected by either growth state or changes in the actual nutrient composition. Even if isolated strains were cultivated in standard nutrient broth for several months, only slight differences in the zeta potential profiles were measured. Only the isoelectric focusing experiments indicated thatcultivation in a complex medium favoured a progressively decreased uniformity of surface charge properties.
Thus, measurement of zeta potential profiles under standardized conditions may be a useful means to compare the surface structures of bacteria from different origins.
|Martienssen, M., Reichel, O., Kohlweyer, U. (2001):
Surface properties of bacteria from different wastewater treatment plants
Acta Biotechnol. 21 (3), 207 - 225