Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) A method for trace element determination of single daphnia specimens using total reflection x-ray fluorescence spectrometry
Autor Mages, M.; Wölfl, S.; von Tümpling, W.;
Journal / Serie Spectrochimica Acta Part B: Atomic Spectroscopy
Erscheinungsjahr 2001
Department FLOEK; GM;
Band/Volume 56
Heft 11
Sprache englisch;
Abstract Two new preparation techniques for total-reflection X-ray fluorescence (TXRF) element determination of single freshwater crustacean specimens (dry weight: 3-40 mug ind(-1)) have been developed and tested using Daphnia pulex from a deep, oligotrophic freshwater lake located in southern Chile. Dry, method: Specimens were washed with 0.2 Rm filtered lake water and frozen in liquid nitrogen. The freeze-dried Daphnia specimens were weighed using an ultra-fine microbalance and placed on quartz glass carriers for TXRF analysis. Wet method: Specimens were washed with 0.2 mum filtered lake water and placed on quartz glass carriers for TXRF analysis and dried in air. The dry weight was determined using the previously established body length-dry weight relationship. Method validation for both the dry and the wet preparation method in combination with TXRF spectrometry for the element determination in small single freshwater crustaceans showed that both methods can be used for routine investigations. There were no significant differences between the dry and the wet methods concerning the elements Ca, K, Fe, Zn, Br, P, Cu, but the determination of Mn, S and Sr revealed significant differences between the two methods. It seems that the dry method yields more precise results, but the wet method is easier to handle in the field when samples cannot be fixed with liquid nitrogen.
ID 6582
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6582
Mages, M., Wölfl, S., von Tümpling, W. (2001):
A method for trace element determination of single daphnia specimens using total reflection x-ray fluorescence spectrometry
Spectroc. Acta Pt. B-Atom. Spectr. 56 (11), 2209 - 2217