Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1007/s002530100808 |
Volltext | Shareable Link |
Titel (primär) | Regiospecific effect of 1-octanol on cis-trans isomerisation of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12 |
Autor | Heipieper, H.J.
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Quelle | Applied Microbiology and Biotechnology |
Erscheinungsjahr | 2001 |
Department | UBT; SAN |
Band/Volume | 57 |
Heft | 4 |
Seite von | 541 |
Seite bis | 547 |
Sprache | englisch |
Abstract | The solvent-tolerant bacterium Pseudomonas putida S12, which adapts its membrane lipids to the presence of toxic solvents by a cis to trans isomerization of unsaturated fatty acids, was used to study possible in vivo regiospecificity of the isomerase. Cells were supplemented with linoleic acid (C18:2Δ9-cis,Δ12-cis), a fatty acid that cannot be synthesized by this bacterium, but which was incorporated into membrane lipids up to an amount of 15% of total fatty acids. After addition of 1-octanol, which was used as an activator of the cis-trans isomerase, the linoleic acid was converted into the Δ9-trans,Δ12-cis isomer, while the Δ9-cis,Δ12-trans and Δ9-trans,Δ12-trans isomers were not synthesized. Thus, for the first time, regiospecific in vivo formation of novel, mixed cis/trans isomers of dienoic fatty acid chains was observed. The maximal conversion (27–36% of the chains) was obtained at 0.03–0.04% (v/v) octanol, after 2 h. The observed regiospecificity of the enzyme, which is located in the periplasmic space, could be due to penetration of the enzyme to a specific depth in the membrane as well as to specific molecular recognition of the substrate molecules. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6402 |
Heipieper, H.J., de Waard, P., van der Meer, P., Killian, J.A., Isken, S., de Bont, J.A.M., Eggink, G., de Wolf, F.A. (2001): Regiospecific effect of 1-octanol on cis-trans isomerisation of unsaturated fatty acids in the solvent-tolerant strain Pseudomonas putida S12 Appl. Microbiol. Biotechnol. 57 (4), 541 - 547 10.1007/s002530100808 |