Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Liquid chromatography—mass spectrometry and liquid chromatography—tandem mass spectrometry determination of N-methylpyrrolidinone in riverine biofilms
Autor Headley, J.V.; Peru, K.M.; Friesen, D.A.; Neu, T.R.;
Journal / Serie Journal of Chromatography A
Erscheinungsjahr 2001
Department FLOEK; GM;
Band/Volume 917
Heft 1-2
Sprache englisch;
Keywords Riverine biofilms
Abstract A rugged procedure utilizing reversed-phase liquid chromatography with positive-ion electrospray ionization mass spectrometry (LC–MS) along with tandem MS is described for the quantification and confirmation of N-methylpyrrolidinone (NMP) in methanolic extracts of riverine biofilm. The LC–MS method provided a 100-fold improvement in detection limits (2 ng g−1 with a repeatability of 80–95% based on triplicate analyses) compared to a conventional LC–UV detection procedure and was applicable to quantitative analysis of biofilm samples with little or no clean up. Under low-energy collision induced dissociation (CID) conditions (17 V, laboratory frame of reference, with argon as the collision gas), two product-ions of the [M+H]+ ion were formed at m/z 69 [MH-CH3NH2]+ and m/z 58 [MH-CH3NCH]+ with relative abundances of 30% and 5%, respectively. These CID transitions were used to demonstrate that biofilm uptake of a photocatalytically-generated mixture of NMP was rapid once acclimation was achieved.
ID 6396
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6396
Headley, J.V., Peru, K.M., Friesen, D.A., Neu, T.R. (2001):
Liquid chromatography—mass spectrometry and liquid chromatography—tandem mass spectrometry determination of N-methylpyrrolidinone in riverine biofilms
J. Chromatogr. A 917 (1-2), 159 - 165