Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1007/s00253-002-1080-y |
Volltext | Shareable Link |
Titel (primär) | Cis/trans isomerisation of unsaturated fatty acids in a cardiolipin synthese knock-out mutant of Pseudomonas putida P8 |
Autor | von Wallbrunn, A.; Heipieper, H.J.
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Quelle | Applied Microbiology and Biotechnology |
Erscheinungsjahr | 2002 |
Department | UBT |
Band/Volume | 60 |
Heft | 1-2 |
Seite von | 179 |
Seite bis | 185 |
Sprache | englisch |
Abstract | The gene encoding cardiolipin synthase (cls) from the phenol-degrading bacterium Pseudomonas putida P8, which rapidly adapts its membrane lipids to the presence of organic solvents by cis/trans isomerisation of unsaturated fatty acids, was isolated and completely sequenced. The functionality of the predicted gene product was proven by constructing a knock-out mutant that was significantly reduced in its growth rate both at elevated temperatures and in the presence of membrane-active solvents. Though the mutant showed a clear phenotype it was still able to synthesise trace amounts of cardiolipin. As an increase in cardiolipin (diphosphatidylglycerol) content is known to function as a long term membrane adaptation mechanism in pseudomonads, we tested whether the mutant compensates for the lack of the Cls by increased cis/trans isomerisation of unsaturated fatty acids. Increase in cis/trans isomerisation of unsaturated fatty acids was observed for the mutant at zero and low concentrations of 4-chlorophenol; however, cis/trans isomerisation is not able to fully compensate for the lack of cardiolipin production. Possibly, other long-term adaptation mechanisms are instrumental in compensating for the missing cardiolipin synthesis. As the cis/trans isomerase is activated similarly in the mutant and the wildtype, cis/trans isomerisation and cardiolipin production do not display mutual dependency. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6104 |
von Wallbrunn, A., Heipieper, H.J., Meinhardt, F. (2002): Cis/trans isomerisation of unsaturated fatty acids in a cardiolipin synthese knock-out mutant of Pseudomonas putida P8 Appl. Microbiol. Biotechnol. 60 (1-2), 179 - 185 10.1007/s00253-002-1080-y |