Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00253-002-1080-y
Volltext Shareable Link
Titel (primär) Cis/trans isomerisation of unsaturated fatty acids in a cardiolipin synthese knock-out mutant of Pseudomonas putida P8
Autor von Wallbrunn, A.; Heipieper, H.J. ORCID logo ; Meinhardt, F.
Quelle Applied Microbiology and Biotechnology
Erscheinungsjahr 2002
Department UBT
Band/Volume 60
Heft 1-2
Seite von 179
Seite bis 185
Sprache englisch
Abstract The gene encoding cardiolipin synthase (cls) from the phenol-degrading bacterium Pseudomonas putida P8, which rapidly adapts its membrane lipids to the presence of organic solvents by cis/trans isomerisation of unsaturated fatty acids, was isolated and completely sequenced. The functionality of the predicted gene product was proven by constructing a knock-out mutant that was significantly reduced in its growth rate both at elevated temperatures and in the presence of membrane-active solvents. Though the mutant showed a clear phenotype it was still able to synthesise trace amounts of cardiolipin. As an increase in cardiolipin (diphosphatidylglycerol) content is known to function as a long term membrane adaptation mechanism in pseudomonads, we tested whether the mutant compensates for the lack of the Cls by increased cis/trans isomerisation of unsaturated fatty acids. Increase in cis/trans isomerisation of unsaturated fatty acids was observed for the mutant at zero and low concentrations of 4-chlorophenol; however, cis/trans isomerisation is not able to fully compensate for the lack of cardiolipin production. Possibly, other long-term adaptation mechanisms are instrumental in compensating for the missing cardiolipin synthesis. As the cis/trans isomerase is activated similarly in the mutant and the wildtype, cis/trans isomerisation and cardiolipin production do not display mutual dependency.
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=6104
von Wallbrunn, A., Heipieper, H.J., Meinhardt, F. (2002):
Cis/trans isomerisation of unsaturated fatty acids in a cardiolipin synthese knock-out mutant of Pseudomonas putida P8
Appl. Microbiol. Biotechnol. 60 (1-2), 179 - 185 10.1007/s00253-002-1080-y