Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) The inducibility of human cytochrome P450 1A by environmental-relevant xenobiotics in the human hepatoma derived cell line HepG2
Autor Rudzok, S.; Schmücking, E.; Graebsch, C.; Herbarth, O.; Bauer, M.;
Journal / Serie Environmental Toxicology and Pharmacology
Erscheinungsjahr 2009
Department IMMU; STUDIEN;
Band/Volume 28
Heft 3
Sprache englisch;
Keywords CYP1A; HepG2; Pesticides; Xenobiotic; Human
Abstract Overexpression of the CYP1 family, independent of gender, is focal to the evaluation of the risk of human cancer. We have analysed the ability of 17 anthropogenic environmental xenobiotics widely used in Europe within households and agriculture to induce the human cytochrome P450 1A (CYP1A) in the human hepatoma derived cell line HepG2.The xenobiotics were potent to concomitantly induce both CYP1A mRNA and CYP1A activity in a dose-response relationship. Exceptions were shown by the organophosphate insecticide chlorpyrifos and the imidazole fungicide prochloraz in high concentrations which were capable of both inhibiting the basal or abolishing the initially induced CYP1A activity, respectively. A CYP1A induction has been shown for the first time by the aromatic xenobiotics irgasan, permethrin and azoxystrobin, the nonaromatic tributyltinoxide and for humans by the piperonylbutoxide.The xenobiotics additionally differed by their induced CYP1A isoenzyme pattern. A pronounced CYP1A1 and CYP1A2 mRNA induction was given by the phenyl urea herbicide diuron and benzodiazole insecticide piperonylbutoxide, respectively.In conclusion, out of the environmental xenobiotics, we described new members of human CYP1A inducers which extend chemical structures of biotransformation activators.
ID 546
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=546
Rudzok, S., Schmücking, E., Graebsch, C., Herbarth, O., Bauer, M. (2009):
The inducibility of human cytochrome P450 1A by environmental-relevant xenobiotics in the human hepatoma derived cell line HepG2
Environ. Toxicol. Pharmacol. 28 (3), 370 - 378