Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Assimilatory detoxification of herbicides by Delftia acidovorans MC1: Induction of two chlorocatechol 1,2-dioxygenases as response to chemostress
Autor Benndorf, D.; Babel, W.;
Journal / Serie Microbiology-SGM
Erscheinungsjahr 2002
Department UMB; PROTEOM;
Band/Volume 148
Heft 9
Sprache englisch;
Keywords resistance mechanism; degradation; chlorophenoxy herbicides; 2D-PAGE
Abstract Proteome analysis of bacteria that can detoxify harmful organic compounds enables the discovery of enzymes involved in the biodegradation of these substances and proteins that protect the cell against poisoning. Exposure of Delftia acidovorans MC1 to 2,4-dichlorophenoxypropionic acid and its metabolites 2,4-dichlorophenol and 3,5-dichlorocatechol during growth on pyruvate as a source of carbon and energy induced several proteins. Contrary to the general hypothesis that lipophilic or reactive compounds induce heat shock or oxidative stress proteins, no induction of the GroEL, DnaK and AhpC proteins that were used as markers for the induction of heat shock and oxidative stress responses was observed. However, two chlorocatechol1,2-dioxygenases, identified by amino terminal sequence analysis, were induced. Both enzymes catalyse the conversion of 3,5-dichlorocatechol to 2,4-dichloro-cis,cis-muconate indicating that biodegradation is a major mechanism of resistance in the detoxifying bacterium D. acidovorans MC1.
ID 5351
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=5351
Benndorf, D., Babel, W. (2002):
Assimilatory detoxification of herbicides by Delftia acidovorans MC1: Induction of two chlorocatechol 1,2-dioxygenases as response to chemostress
Microbiology-(UK) 148 (9), 2883 - 2888