Details zur Publikation |
Kategorie | Textpublikation |
Referenztyp | Zeitschriften |
DOI | 10.1264/jsme2.19.1 |
Titel (primär) | Two-photon imaging for studying the microbial ecology of biofilm systems |
Autor | Neu, T.R.; Walczysko, P.; Lawrence, J.R. |
Quelle | Microbes and Environments |
Erscheinungsjahr | 2004 |
Department | FLOEK |
Band/Volume | 19 |
Heft | 1 |
Seite von | 1 |
Seite bis | 6 |
Sprache | englisch |
Abstract | Conventional confocal laser scanning microscopy uses continuous wave visible or UV lasers for one-photon excitation and imaging. These instruments are now enhanced with pulsed infrared lasers allowing two-photon excitation. With the appropriate technical setup two-photon excitation may be used for intensity imaging or for lifetime imaging. Both of these techniques have specific advantages if compared with one-photon excitation. Despite this fact, to date mainly one-photon excitation has been employed in microbial ecology. In this review the potential of two-photon intensity and lifetime imaging is discussed, using appropriate examples from cell biology as well as the first reports in microbiology. |
dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=4394 |
Neu, T.R., Walczysko, P., Lawrence, J.R. (2004): Two-photon imaging for studying the microbial ecology of biofilm systems Microbes Environ. 19 (1), 1 - 6 10.1264/jsme2.19.1 |