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Titel (primär) Studies on hydrogenase activity and chlorobenzene respiration in Dehalococcoides sp. strain CBDB1
Autor Jayachandran, G.; Görisch, H.; Adrian, L.;
Journal / Serie Archives of Microbiology
Erscheinungsjahr 2004
Department ISOBIO;
Band/Volume 182
Heft 6
Sprache englisch;
Keywords Dehalococcoides; Chlorobenzenes; Reductive dechlorination; Dehalorespiration; Hydrogenase
Abstract Hydrogen oxidation and electron transport were studied in the chlorobenzene-utilizing anaerobe Dehalococcoides sp. strain CBDB1. While Cu2+ and Hg2+ ions irreversibly inhibited hydrogenase activity in intact cells, Ni2+ ions inhibited reversibly. About 80% of the initial hydrogenase activity was inactivated within 30 s when the cells were exposed to air. In contrast, hydrogenase was active at a redox potential of +10 mV when this redox potential was established anoxically with a redox indicator. Viologen dyes served both as electron acceptor for hydrogenase and electron donor for the dehalogenase. A menaquinone analogue, 2,3-dimethyl 1,4-naphthoquinone, served neither as electron acceptor for the hydrogenase nor as electron donor for the dehalogenase. In addition, the menaquinone antagonist 2-n-heptyl-4-hydroxyquinoline-N-oxide had no effect on dechlorination catalyzed by cell suspensions or isolated membranes with hydrogen as electron donor, lending further support to the notion that menaquinone is not involved in electron transport. The ionophores tetrachlorosalicylanilide and carbonylcyanide m-chlorophenylhydrazone did not inhibit dechlorination by cell suspensions, indicating that strain CBDB1 does not require reverse electron transport. The ATP-synthase inhibitor N,Nprime-dicyclohexylcarbodiimide inhibited the dechlorination reaction with cell suspensions; however, the latter effect was partially relieved by the addition of tetrachlorosalicylanilide. 1,2,3,4-Tetrachlorobenzene strongly inhibited dechlorination of other chlorobenzenes by cell suspensions with hydrogen as electron donor, but it did not interfere with either hydrogenase or dehalogenase activity.
ID 4211
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Jayachandran, G., Görisch, H., Adrian, L. (2004):
Studies on hydrogenase activity and chlorobenzene respiration in Dehalococcoides sp. strain CBDB1
Arch. Microbiol. 182 (6), 498 - 504