Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Growth on phenol at chemostress levels amplifies the expression of the phenol degradation pathway in Acinetobacter calcoaceticus
Autor Benndorf, D.; Loffhagen, N.; Härtig, C.; Babel, W.;
Journal / Serie Engineering in Life Sciences
Erscheinungsjahr 2004
Department UMB; PROTEOM;
Band/Volume 4
Heft 1
Sprache englisch;
Keywords Degradation; Enzymaic processes; Microorganisms; Organic pollution
Abstract Bacteria often use pollutants as sole carbon and energy sources. However, if they are toxic and the concentrations are high these compounds inhibit growth, and eventually poison the biocatalysts. In order to identify mechanisms contributing to stability, the protein patterns of Acinetobacter calcoaceticus during growth on potentially toxic phenol were analysed using two-dimensional gel electrophoresis. Enzymes involved in the catabolism or assimilation of phenol, such as phenol monooxygenase and catechol 1,2-dioxygenase, were induced at a more than twofold level in response to long-term exposure to high concentrations of the compound serving as the sole carbon and energy source. This would have clear adaptive benefits, since increased rates of consumption of phenol would reduce the susceptibility of Acinetobacter calcoaceticus to phenol poisoning. In contrast, transient induction of only one heat shock protein and one oxidative stress protein was detected during long-term exposure to high concentrations of phenol.
ID 3970
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3970
Benndorf, D., Loffhagen, N., Härtig, C., Babel, W. (2004):
Growth on phenol at chemostress levels amplifies the expression of the phenol degradation pathway in Acinetobacter calcoaceticus
Eng. Life Sci. 4 (1), 38 - 42