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Referenztyp Zeitschriften
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Titel (primär) Supersensitivity of P2X7 receptors in cerebrocortical cell cultures after in vitro ischemia
Autor Wirkner, K.; Köfalvi, A.; Fischer, W.; Günther, A.; Franke, H.; Gröger-Arndt, H.; Nörenberg, W.; Madarász, E.; Vizi, E.S.; Schneider, D.; Sperlágh, B.; Illes, P.;
Journal / Serie Journal of Neurochemistry
Erscheinungsjahr 2005
Department PROTEOM;
Band/Volume 95
Heft 5
Sprache englisch;
Abstract Neuronally enriched primary cerebrocortical cultures were exposed to glucose- free medium saturated with argon (in vitro ischemia) instead of oxygen ( normoxia). Ischemia did not alter P2X(7) receptor mRNA, although serum deprivation clearly increased it. Accordingly, P2X(7) receptor immunoreactivity (IR) of microtubuline- associated protein 2 (MAP2)- IR neurons or of glial fibrillary acidic protein (GFAP)- IR astrocytes was not affected; serum deprivation augmented the P2X(7) receptor IR only in the astrocytic, but not the neuronal cell population. However, ischemia markedly increased the ATP- and 2 '- 3 '-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (BzATP)- induced release of previously incorporated [(3) H] GABA. Both Brilliant Blue G and oxidized ATP inhibited the release of [(3) H] GABA caused by ATP application; the Brilliant Blue G- sensitive, P2X(7) receptor-mediated fraction, was much larger after ischemia than after normoxia. Whereas ischemic stimulation failed to alter the amplitude of ATP- and BzATP- induced small inward currents recorded from a subset of non- pyramidal neurons, BzATP caused a more pronounced increase in the frequency of miniature inhibitory postsynaptic currents (mIPSCs) after ischemia than after normoxia. Brilliant Blue G almost abolished the effect of BzATP in normoxic neurons. Since neither the amplitude of mIPSCs nor that of the muscimol- induced inward currents was affected by BzATP, it is assumed that BzATP acts at presynaptic P2X(7) receptors. Finally, P2X(7) receptors did not enhance the intracellular free Ca2+ concentration either in proximal dendrites or in astrocytes, irrespective of the normoxic or ischemic preincubation conditions. Hence, facilitatory P2X(7) receptors may be situated at the axon terminals of GABAergic non-pyramidal neurons. When compared with normoxia, ischemia appears to markedly increase P2X(7) receptor-mediated GABA release, which may limit the severity of the ischemic damage. At the same time we did not find an accompanying enhancement of P2X(7) mRNA or protein expression, suggesting that receptors may become hypersensitive because of an increased efficiency of their transduction pathways
ID 3906
dauerhafte UFZ-Verlinkung
Wirkner, K., Köfalvi, A., Fischer, W., Günther, A., Franke, H., Gröger-Arndt, H., Nörenberg, W., Madarász, E., Vizi, E.S., Schneider, D., Sperlágh, B., Illes, P. (2005):
Supersensitivity of P2X7 receptors in cerebrocortical cell cultures after in vitro ischemia
J. Neurochem. 95 (5), 1421 - 1437