Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences
Autor Nikolausz, M.; Kappelmeyer, U.; Nijenhuis, I.; Ziller, K.; Kästner, M.;
Journal / Serie Systematic and Applied Microbiology
Erscheinungsjahr 2005
Department ISOBIO; UBT;
Band/Volume 28
Heft 7
Sprache englisch;
Keywords Hyphomicrobium; Dichloromethane; 16S rDNA; dcmA; Dehalogenase
Abstract A phylogenetic analysis of 6 strains of dichloromethane (DCM) utilizing bacteria was performed. Based on the almost complete 16S rDNA sequence determination, all strains clustered together and showed high sequence similarity to Hyphomicrobium denitrificans, except for the strain MC8b, which is only moderately related to them and probably represents a distinct species. The 16S rDNA-based phylogenetic tree was compared to the one obtained from the DNA sequence data of the dcmA gene coding DCM dehalogenase, the key enzyme of DCM utilization. The topology of the two trees is in good agreement and may suggest an ancient origin of DCM dehalogenase, but also raises questions about the original role of the enzyme.
ID 3654
dauerhafte UFZ-Verlinkung http://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=3654
Nikolausz, M., Kappelmeyer, U., Nijenhuis, I., Ziller, K., Kästner, M. (2005):
Molecular characterization of dichloromethane-degrading Hyphomicrobium strains using 16S rDNA and DCM dehalogenase gene sequences
Syst. Appl. Microbiol. 28 (7), 582 - 587