Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00792-005-0431-x
Volltext Shareable Link
Titel (primär) Cells of Pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size
Autor Neumann, G.; Veeranagouda, Y.; Karegoudar, T.B.; Sahin, Ö.; Mäusezahl, I.; Kabelitz, N.; Kappelmeyer, U. ORCID logo ; Heipieper, H.J. ORCID logo
Quelle Extremophiles
Erscheinungsjahr 2005
Department UBT
Band/Volume 9
Heft 2
Seite von 163
Seite bis 168
Sprache englisch
Abstract The phenol-degrading solvent-tolerant bacterium Pseudomonas putida P8 changed its cell shape when grown in the presence of aromatic compounds such as phenol and 4-chlorophenol. The sizes of cells that had been growing after addition of different concentrations of the toxic compounds were measured using a coulter counter that calculates the sizes of the rod-shaped bacteria to diameters of virtual spheres. The cells showed an increase in the diameter depending on the toxic effects of the applied concentrations of both solvents. The same effect was measured for an alkanol degrading bacterium, Enterobacter sp. VKGH12, in the presence of n-butanol. The reaction of the cells to different concentrations of n-butanol was examined by scanning electron microscopy. With this technique it could be shown that the size of the bacteria increased with increasing concentrations of n-butanol. These changes in cell size were dependent on the cellular activity and occurred only after addition of non-lethal concentrations. In the presence of lethal concentrations that completely inhibited cell growth, the cell sizes were similar to those of cells without intoxication. Taking into account the mathematical formula for spherical and cylindrical diameter and surface, respectively, the cells reacted to the presence of organic solvents by decreasing the ratio between surface and volume of the cells and therefore reducing their relative surfaces. As the cell surface and especially the cytoplasmic membrane are the major targets for the toxic effects of membrane-active compounds, this reduction of the relative surface represents an adaptive response to the presence of such compounds.

dauerhafte UFZ-Verlinkung
Neumann, G., Veeranagouda, Y., Karegoudar, T.B., Sahin, Ö., Mäusezahl, I., Kabelitz, N., Kappelmeyer, U., Heipieper, H.J. (2005):
Cells of Pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size
Extremophiles 9 (2), 163 - 168 10.1007/s00792-005-0431-x