Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/s5060411
Titel (primär) Voltammetric detection of damage to DNA by arsenic compounds at a DNA biosensor
Autor Labuda, J.; Bubnicova, K.; Kovalova, L.; Vanickova, M.; Mattusch, J.; Wennrich, R.
Journal / Serie Sensors
Erscheinungsjahr 2005
Department ANA
Band/Volume 5
Heft 6-10
Seite von 411
Seite bis 423
Sprache englisch

DNA biosensor can serve as a powerfull tool for simple in vitro tests of chemical toxicity. In this paper, damage to DNA attached to the surface of screen-printed carbon electrode by arsenic compounds in solution is described. Using the Co(III) complex with 1,10-phenanthroline, [Co( phen)(3)](3+), as an electrochemical DNA marker and the Ru(II) complex with bipyridyne, [Ru( bipy)(3)](2+), as a DNA oxidation catalyst, the portion of original dsDNA which survives an incubation of the biosensor in the cleavage medium was evaluated. The model cleavage mixture was composed of an arsenic compound at 10(-3) mol/L concentration corresponding to real contaminated water, 2x10(-4) mol/L Fe( II) or Cu(II) ions as the redox catalyst, and 1.5x10(-2) mol/L hydrogen peroxide. DNA damage by arsenite, dimethylarsinic acid as the metabolic product of inorganic arsenic and widely used herbicide, as well as phenylarsonic acid and p-arsanilic acid as the representatives of feed additives was found in difference to arsenate.
dauerhafte UFZ-Verlinkung
Labuda, J., Bubnicova, K., Kovalova, L., Vanickova, M., Mattusch, J., Wennrich, R. (2005):
Voltammetric detection of damage to DNA by arsenic compounds at a DNA biosensor
Sensors 5 (6-10), 411 - 423