Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1007/s00294-005-0564-6
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Titel (primär) Two phylogenetically highly distinct β-tubulin genes of the basidiomycete Suillus bovinus
Autor Juuti, J.T.; Jokela, S.; Tarkka, M.T.; Paulin, L.; Lahdensalo, J.
Quelle Current Genetics
Erscheinungsjahr 2005
Department BOOEK
Band/Volume 47
Heft 4
Seite von 253
Seite bis 263
Sprache englisch
Keywords Suillus bovinus; beta-tubulin; expression analysis; phylogeny; intron; ectomycorrhiza
Abstract Genes tubb1 and tubb2 which encode beta-tubulins 1 and 2, respectively, were characterised from the ectomycorrhizal basidiomycete Suillus bovinus. The two beta-tubulins are surprisingly divergent, with the lowest known sequence identity (60%) in any single fungal species. Comparative analysis showed that beta-tubulin 1 and the intron distribution within the tubb1 gene resemble the other beta-tubulins. beta-Tubulin 2, in contrast, is the most divergent fully described fungal beta-tubulin and the gene contains at least 21 introns, which is the largest amount known for any beta-tubulin gene. Despite this divergence, both genes are constitutively expressed in the functional compartments of the mycorrhizosphere and in pure cultures. Transcription of tubb1 is about 2.4 times higher than that of tubb2; and this difference is also seen at the translation level. Evidence suggested that phosphorylation may be the main post-translational modi. cation of both beta-tubulins. The putative GTP-binding site residues of beta-tubulin 1 match crystallised pig beta-tubulin residues, while five of the nine differences in beta-tubulin 2 match the pig alpha-tubulin GTP-site, suggesting the presence of adaptive sequence evolution. In a Bayesian analysis, beta-tubulin 1 joins the other basidiomycete sequences, while beta-tubulin 2 loosely associates with the group of divergent ascomycete sequences without any clear relative among the known full-length fungal beta-tubulin sequences.
dauerhafte UFZ-Verlinkung
Juuti, J.T., Jokela, S., Tarkka, M.T., Paulin, L., Lahdensalo, J. (2005):
Two phylogenetically highly distinct β-tubulin genes of the basidiomycete Suillus bovinus
Curr. Genet. 47 (4), 253 - 263 10.1007/s00294-005-0564-6