Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Assessing contamination levels of Laguna Lake sediments (Philippines) using a contact assay with zebrafish (Danio rerio) embryos
Autor Hallare, A.V.; Kosmehl, T.; Schulze, T.; Hollert, H.; Köhler, H.-R.; Triebskorn, R.;
Journal / Serie Science of the Total Environment
Erscheinungsjahr 2005
Department WANA;
Band/Volume 347
Heft 1-3
Sprache englisch;
Keywords Laguna Lake; Sediment toxicity; Embryotoxicity; Heat shock proteins; Perylene; PAHs; Heavy metals; Zebrafish; Danio rerio; Bioavailability
Abstract The present study investigated the suitability of a sediment contact assay using zebrafish (Danio rerio) embryos to evaluate the degree of lake sediment contamination. As endpoints, developmental parameters (mortality, abnormality, heart rate, and hatching rate) as well as stress protein responses (hsp 70 levels) in the developing embryos were recorded during a 96-h exposure. Fertilized zebrafish eggs were exposed to both the whole as well as organic extract concentrations prepared from collected sediments from 5 sites along Laguna Lake, Philippines. Compared to whole sediment exposure, more severe embryotoxic and teratogenic responses were elicited in embryos exposed to organic extracts. However, since whole sediment-exposed embryos also revealed significant developmental defects, this exposure phase served as the more realistic exposure scenario in our study. Weak to strong upregulation of hsp 70 levels was also registered among embryos exposed to both whole sediments and organic extracts. The observed embryotoxic and proteotoxic responses by zebrafish embryos to Laguna Lake sediment exposures were discussed in relation to the analyzed contaminants in the sediments (heavy metals, PAHs (perylene), solvent). Overall, the present study points out that the sediment contact assay with zebrafish embryos offers a practicable and highly sensitive bioassay for the general assessment of sediment toxicity.
ID 3397
dauerhafte UFZ-Verlinkung
Hallare, A.V., Kosmehl, T., Schulze, T., Hollert, H., Köhler, H.-R., Triebskorn, R. (2005):
Assessing contamination levels of Laguna Lake sediments (Philippines) using a contact assay with zebrafish (Danio rerio) embryos
Sci. Total Environ. 347 (1-3), 254 - 271