Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1128/msystems.00491-26 |
Lizenz  |
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| Titel (primär) | Establishment of an in vitro aerobic bacterial community as a model of the human lung microbiome |
| Autor | Kulosa, M.; Belisário-Ferrari, M.R.; Semmler, F.; Büttner, M.; Duck, C.; Namazi, Z.; Jehmlich, N.
; von Bergen, M.; Bonitz, T.; Kaysser, L. |
| Quelle | mSystems |
| Erscheinungsjahr | 2026 |
| Department | MOLTOX |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Daten-/Softwarelinks | https://doi.org/10.5281/zenodo.21510789 |
| Supplements | Supplement 1 |
| Keywords | microbiome; lung microbiome; microbiology; bacterial consortia; artificial microbiome; co-culture model |
| Abstract | The human lung microbiome is increasingly recognized as a key player in drug metabolism, yet it remains largely understudied. To replicate this complex physiological environment in a controlled setting, we developed a simplified artificial lung microbiome model composed of four representative bacterial species: Pseudomonas koreensis, Rothia aeria, Neisseria cinerea, and Streptococcus downei. We successfully established a stable 10-day co-culture at 34°C using brain heart infusion medium, as validated by quantitative PCR, viability PCR, and conventional microbiological methodologies. Integrated bioinformatic analyses revealed a variety of potential microbe-microbe interactions, which were supported by metaproteomic analysis using mass spectrometry. Our model provides a foundation for in-depth studies, such as, for example, the effects of pulmonary drugs on the lung microbiome, and how, in turn, the microbiome may influence therapeutic outcomes. |
| Kulosa, M., Belisário-Ferrari, M.R., Semmler, F., Büttner, M., Duck, C., Namazi, Z., Jehmlich, N., von Bergen, M., Bonitz, T., Kaysser, L. (2026): Establishment of an in vitro aerobic bacterial community as a model of the human lung microbiome mSystems 10.1128/msystems.00491-26 |
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