Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.ecoenv.2026.120664 |
Lizenz  |
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| Titel (primär) | Impairment of placental function and maternal-fetal immunity cross-talk by per- and polyfluoroalkyl substances during early pregnancy |
| Autor | Xia, Y.; Ayuk, H.S.; Myhre, O.; Zenclussen, A.C.; Stojanovska, V. |
| Quelle | Ecotoxicology and Environmental Safety |
| Erscheinungsjahr | 2026 |
| Department | IMMU |
| Band/Volume | 323 |
| Seite von | art. 120664 |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Supplements | Supplement 1 Supplement 2 |
| Keywords | Trophoblast spheroids; PFAS; Endocrine disrupting chemicals (EDCs); CD4+ T cells; Maternal-fetal interface |
| Abstract | Per- and
polyfluoroalkyl substances (PFAS) are persistent environmental
pollutants that have been associated with adverse pregnancy outcomes and
immune dysfunction. However, the impact of PFAS on trophoblast-mediated
immune interactions at the maternal-fetal interface remains unclear. In
this study, we evaluated the effects of human-relevant PFAS on
trophoblast function and immune crosstalk using a three-dimensional (3D)
trophoblast spheroid model and a co-culture system with CD4+
T cells. JEG-3 spheroids were exposed to multiple PFAS, including
perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA),
perfluorohexane sulfonate (PFHxS), perfluorononanoic acid (PFNA),
perfluorodecanoic acid (PFDA), and perfluoroundecanoic acid (PFUnDA),
individually and as a mixture at real-life concentrations to assess
viability, invasion and trophoblast-derived β-hCG secretion. Using a
placental–immune cell co-culture model, we examined bidirectional
interactions and their respective effects on functional endpoints in
both cellular compartments and further investigated how exposure to PFAS
may interfere in the bidirectional communication. We reported a
significant decrease in trophoblast viability, after exposure to
individual PFOS, PFOA and the PFAS mixture. PFAS mixture, PFOS, PFHxS,
PFNA and PFDA exposure significantly increased invasiveness in JEG-3
spheroids, while PFOA decreased JEG-3 invasion significantly.
Additionally, β-hCG production declined after 48 h of PFOS and PFAS
mixture exposure in JEG-3 spheroids. Moreover, following PFAS mixture
exposure, we observed a downregulation of CD69 and IFNγ production in
CD4+ T cells, particularly within Th2 cells, while CD154
expression was also reduced. Overall, these findings suggest that PFAS
exposure may influence trophoblast-mediated immune signaling, with
potential implications for pregnancy-related processes. |
| Xia, Y., Ayuk, H.S., Myhre, O., Zenclussen, A.C., Stojanovska, V. (2026): Impairment of placental function and maternal-fetal immunity cross-talk by per- and polyfluoroalkyl substances during early pregnancy Ecotox. Environ. Safe. 323 , art. 120664 10.1016/j.ecoenv.2026.120664 |
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