Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.55003/cast.2025.265614 |
Lizenz  |
|
| Titel (primär) | Enzymes involved in lignocellulose decomposition and effects of fungal pretreatment during solid-state fermentation of lignocellulosic agricultural residues: The case of Trametes versicolor |
| Autor | Duong, H.L.; Schlosser, D.
|
| Quelle | Current Applied Science and Technology |
| Erscheinungsjahr | 2025 |
| Department | AME |
| Seite von | e026561 |
| Sprache | englisch |
| Topic | T7 Bioeconomy |
| Keywords | crude extract; fungal pretreatment; ligninolytic fungi; lignocellulose;Trametes versicolor |
| Abstract | Ligninolytic fungi are considered useful for different
biotechnological uses such as lignocellulose pretreatment for
biorefinery applications, composting, mushroom production, or
bioremediation purposes. Enzymes contributing to lignocellulose
breakdown represent valuable biocatalysts. Trametes versicolor
is a well-known ligninolytic white-rot basidiomycete capable of growing
on different lignocellulosic materials. In the present study, the use of
T. versicolor, which secretes several enzymes capable of
degrading lignocellulosic biomass, was evaluated from two
biotechnological aspects including fungal crude extracts and effects on
enzymatic saccharification of wheat straw. We used 20-day-old wheat
straw cultures of this fungus to characterize its
lignocellulose-decomposing multi-enzyme complex. At the end of the
cultivation period, losses of approximately 41% in lignin and 30% in
total dry mass were observed relative to their initial values. A total
of 17 enzyme activities, each acting differently on the polysaccharide
components of the lignocellulosic substrate, and lignin-modifying enzyme
activities (laccase, peroxidases) were investigated in aqueous extracts
of fungal cultures. The activities of carboxylesterase (substrate:
4-nitrophenyl valerate), endo-1,4-β-D-glucanase (substrate:
2-hydroxyethylcellulose), and laccase were found to predominate. In
summary, fungal crude extracts containing different enzyme activities
may be promising for various biotechnological purposes. Trametes versicolor effectively pretreated wheat straw, significantly increasing reducing sugar levels compared to controls. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=31289 |
| Duong, H.L., Schlosser, D. (2025): Enzymes involved in lignocellulose decomposition and effects of fungal pretreatment during solid-state fermentation of lignocellulosic agricultural residues: The case of Trametes versicolor Curr. Appl. Sci. Technol. , e026561 10.55003/cast.2025.265614 |
|