Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.rhisph.2025.101062 |
Lizenz  |
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| Titel (primär) | High-resolution sampling for enhanced spatial analysis of microbial growth and enzyme activity in the rhizosphere |
| Autor | Ghaderi, N.; Ibrahim, Z.; Guber, A.; Khosrozadeh, S.; Guliyev, V.; Tarkka, M.; Blagodatskaya, E. |
| Quelle | Rhizosphere |
| Erscheinungsjahr | 2025 |
| Department | BOOEK |
| Band/Volume | 34 |
| Seite von | art. 101062 |
| Sprache | englisch |
| Topic | T5 Future Landscapes |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S2452219825000473-mmc1.docx |
| Keywords | Hydrolytic enzymes; Maize; Microplate assay; Microbial biomass; Time-lapse zymography |
| Abstract | The rhizosphere is a hotspot of microbial activity and enzymatic processes driven by concentration gradients of root exudates. Traditional methods based on destructive sampling often lack the sensitivity required to accurately reflect the spatial gradients of microbial activities within the rhizosphere. This is often identified by the discrepancies between microplate assays and zymography. We addressed this methodological gap through a comparative study of the spatial dynamics of enzymatic processes in the maize rhizosphere. Two genotypes of Zea mays L.: wild type and root hair defective rth3 mutant were used to test the discrepancies between microplate assay and zymography. First, enzyme activity was mapped using zymography, followed by destructive sampling (<1 mm, 1–2 mm, and >2 mm) for microplate assay. In addition, we tested the microbial growth kinetics across spatial gradients (<2 mm, >2 mm, and non-rhizosphere soil). The 1 mm sampling revealed significant rhizosphere gradients in microplate assay, particularly for β-glucosidase, with a gradual decrease in Vmax at 1–2 mm (up to 1.7 times) and >2 mm (up to 4.5 times) compared to <1 mm. The observed enzyme activity gradients correlated positively with active microbial biomass and CO2 emission rates. Active biomass was up to 29 times greater at <2 mm compared to >2 mm. The lag-time before growth was 0.5 h shorter at <2 mm than at >2 mm. This study highlights the need for short-distance sampling techniques to accurately capture the spatial distribution of microbial growth and enzyme activity in the rhizosphere. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=30631 |
| Ghaderi, N., Ibrahim, Z., Guber, A., Khosrozadeh, S., Guliyev, V., Tarkka, M., Blagodatskaya, E. (2025): High-resolution sampling for enhanced spatial analysis of microbial growth and enzyme activity in the rhizosphere Rhizosphere 34 , art. 101062 10.1016/j.rhisph.2025.101062 |
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