Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1038/s41586-024-07832-z
Lizenz creative commons licence
Titel (primär) Growth of complete ammonia oxidizers on guanidine
Autor Palatinszky, M.; Herbold, C.W.; Sedlacek, C.J.; Pühringer, D.; Kitzinger, K.; Giguere, A.T.; Wasmund, K.; Nielsen, P.H.; Dueholm, M.K.D.; Jehmlich, N. ORCID logo ; Gruseck, R.; Legin, A.; Kostan, J.; Krasnici, N.; Schreiner, C.; Palmetzhofer, J.; Hofmann, T.; Zumstein, M.; Djinović-Carugo, K.; Daims, H.; Wagner, M.
Quelle Nature
Erscheinungsjahr 2024
Department MOLTOX
Band/Volume 633
Heft 8030
Seite von 646
Seite bis 653
Sprache englisch
Topic T9 Healthy Planet
Daten-/Softwarelinks https://doi.org/10.15151/ESRF-DC-1801440672F-DC-1801440672
https://doi.org/10.6084/m9.figshare.26139127.v2
Supplements https://static-content.springer.com/esm/art%3A10.1038%2Fs41586-024-07832-z/MediaObjects/41586_2024_7832_MOESM4_ESM.xlsx
https://static-content.springer.com/esm/art%3A10.1038%2Fs41586-024-07832-z/MediaObjects/41586_2024_7832_MOESM5_ESM.xlsx
Abstract Guanidine is a chemically stable nitrogen compound that is excreted in human urine and is widely used in manufacturing of plastics, as a flame retardant and as a component of propellants, and is well known as a protein denaturant in biochemistry. Guanidine occurs widely in nature and is used by several microorganisms as a nitrogen source, but microorganisms growing on guanidine as the only substrate have not yet been identified. Here we show that the complete ammonia oxidizer (comammox) Nitrospira inopinata and probably most other comammox microorganisms can grow on guanidine as the sole source of energy, reductant and nitrogen. Proteomics, enzyme kinetics and the crystal structure of a N. inopinata guanidinase homologue demonstrated that it is a bona fide guanidinase. Incubation experiments with comammox-containing agricultural soil and wastewater treatment plant microbiomes suggested that guanidine serves as substrate for nitrification in the environment. The identification of guanidine as a growth substrate for comammox shows an unexpected niche of these globally important nitrifiers and offers opportunities for their isolation
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29631
Palatinszky, M., Herbold, C.W., Sedlacek, C.J., Pühringer, D., Kitzinger, K., Giguere, A.T., Wasmund, K., Nielsen, P.H., Dueholm, M.K.D., Jehmlich, N., Gruseck, R., Legin, A., Kostan, J., Krasnici, N., Schreiner, C., Palmetzhofer, J., Hofmann, T., Zumstein, M., Djinović-Carugo, K., Daims, H., Wagner, M. (2024):
Growth of complete ammonia oxidizers on guanidine
Nature 633 (8030), 646 - 653 10.1038/s41586-024-07832-z