Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.3791/66153 |
Lizenz  |
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| Titel (primär) | Evaluating toxicity of chemicals using a zebrafish vibration startle response screening system |
| Autor | Hayot, G.; Marcato, D.; Cramer von Clausbruch, C.A.; Pace, G.; Strähle, U.; Colbourne, J.K.; Pylatiuk, C.; Peravali, R.; Weiss, C.; Scholz, S.
; Dickmeis, T. |
| Quelle | JoVe-Journal of Visualized Experiments |
| Erscheinungsjahr | 2024 |
| Department | ETOX |
| Band/Volume | 2024 |
| Heft | 203 |
| Seite von | e66153 |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://www.jove.com/files/ftp_upload/66153/Supplementary File 1.zip https://www.jove.com/files/ftp_upload/66153/Supplementary File 2.zip https://www.jove.com/files/ftp_upload/66153/Supplementary File 3.zip |
| Abstract | We developed a simple screening system for the evaluation of neuromuscular and general toxicity in zebrafish embryos. The modular system consists of electrodynamic transducers above which tissue culture dishes with embryos can be placed. Multiple such loudspeaker-tissue culture dish pairs can be combined. Vibrational stimuli generated by the electrodynamic transducers induce a characteristic startle and escape response in the embryos. A belt-driven linear drive sequentially positions a camera above each loudspeaker to record the movement of the embryos. In this way, alterations to the startle response due to lethality or neuromuscular toxicity of chemical compounds can be visualized and quantified. We present an example of the workflow for chemical compound screening using this system, including the preparation of embryos and treatment solutions, operation of the recording system, and data analysis to calculate benchmark concentration values of compounds active in the assay. The modular assembly based on commercially available simple components makes this system both economical and flexibly adaptable to the needs of particular laboratory setups and screening purposes. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=29402 |
| Hayot, G., Marcato, D., Cramer von Clausbruch, C.A., Pace, G., Strähle, U., Colbourne, J.K., Pylatiuk, C., Peravali, R., Weiss, C., Scholz, S., Dickmeis, T. (2024): Evaluating toxicity of chemicals using a zebrafish vibration startle response screening system J. Vis. Exp. 2024 (203), e66153 10.3791/66153 |
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