Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.taap.2024.116868
Titel (primär) Branching morphogenesis of the mouse mammary gland after exposure to benzophenone-3
Autor Schierano-Marotti, G.; Altamirano, G.A.; Oddie, S.; Gomez, A.L.; Meyer, N.; Muñoz-de-Toro, M.; Zenclussen, A.C.; Rodríguez, H.A.; Kass, L.
Quelle Toxicology and Applied Pharmacology
Erscheinungsjahr 2024
Department IMMU
Band/Volume 484
Seite von art. 116868
Sprache englisch
Topic T9 Healthy Planet
Keywords Branching morphogenesis; Mammary gland; Benzophenone-3; 3D culture; Progesterone receptor; Hormonal regulation
Abstract Pubertal mammary branching morphogenesis is a hormone-regulated process susceptible to exposure to chemicals with endocrine disruptive capacity, such as the UV-filter benzophenone-3 (BP3). Our aim was to assess whether intrauterine or in vitro exposure to BP3 modified the branching morphogenesis of the female mouse mammary gland. For this, pregnant mice were dermally exposed to BP3 (0.15 or 50 mg/kg/day) from gestation day (GD) 8.5 to GD18.5. Sesame oil treatment served as control. Changes of the mammary glands of the offspring were studied on postnatal day 45. Further, mammary organoids from untreated mice were cultured under branching induction conditions and exposed for 9 days to BP3 (1 × 10−6 M, 1 × 10−9 M, or 1 × 10−12 M with 0.01% ethanol as control) to evaluate the branching progression. Mice that were exposed to BP3 in utero showed decreased mRNA levels of progesterone receptor (PR) and WNT4. However, estradiol and progesterone serum levels, mammary histomorphology, proliferation, and protein expression of estrogen receptor alpha (ESR1) and PR were not significantly altered. Interestingly, direct exposure to BP3 in vitro also decreased the mRNA levels of PR, RANKL, and amphiregulin without affecting the branching progression. Most effects were found after exposure to 50 mg/kg/day or 1 × 10−6 M of BP3, both related to sunscreen application in humans. In conclusion, exposure to BP3 does not impair mammary branching morphogenesis in our models. However, BP3 affects PR transcriptional expression and its downstream mediators, suggesting that exposure to BP3 might affect other developmental stages of the mammary gland.
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Schierano-Marotti, G., Altamirano, G.A., Oddie, S., Gomez, A.L., Meyer, N., Muñoz-de-Toro, M., Zenclussen, A.C., Rodríguez, H.A., Kass, L. (2024):
Branching morphogenesis of the mouse mammary gland after exposure to benzophenone-3
Toxicol. Appl. Pharmacol. 484 , art. 116868 10.1016/j.taap.2024.116868