Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Preprints |
| DOI | 10.1101/2024.01.24.576656 |
Lizenz  |
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| Titel (primär) | Stable Isotope Probing-nanoFTIR for quantitation of cellular metabolism and observation of growth-dependent spectral features |
| Autor | Burr, D.J.; Drauschke, J.; Kanevche, K.; Kümmel, S.; Stryhanyuk, H.; Heberle, J.; Perfumo, A.; Elsaesser, A. |
| Quelle | bioRxiv |
| Erscheinungsjahr | 2024 |
| Department | TECH |
| Sprache | englisch |
| Topic | T7 Bioeconomy |
| Abstract | This study utilizes nanoscale Fourier transform infrared spectroscopy (nanoFTIR) to perform stable isotope probing (SIP) on individual bacteria cells cultured in the presence of 13C-labelled glucose. SIP-nanoFTIR simultaneously quantifies single-cell metabolism through infrared spectroscopy and acquires cellular morphological information via atomic force microscopy. The redshift of the amide I peak corresponds to the isotopic enrichment of newly synthesized proteins. These observations of single-cell translational activity are comparable to those of conventional methods, examining bulk cell numbers. Observing cells cultured under conditions of limited carbon, SIP-nanoFTIR is used to identify environmentally-induced changes in metabolic heterogeneity and cellular morphology. Individuals outcompeting their neighboring cells will likely play a disproportionately large role in shaping population dynamics during adverse conditions or environmental fluctuations. Additionally, SIP-nanoFTIR enables the spectroscopic differentiation of specific cellular growth phases. During cellular replication, subcellular isotope distribution becomes more homogenous, which is reflected in the spectroscopic features dependent on the extent of 13C-13C mode coupling or to specific isotopic symmetries within protein secondary structures. As SIP-nanoFTIR captures single-cell metabolism, environmentally-induced cellular processes and subcellular isotope localization, this technique offers widespread applications across a variety of disciplines including microbial ecology, biophysics, biopharmaceuticals, medicinal science and cancer research. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28721 |
| Burr, D.J., Drauschke, J., Kanevche, K., Kümmel, S., Stryhanyuk, H., Heberle, J., Perfumo, A., Elsaesser, A. (2024): Stable Isotope Probing-nanoFTIR for quantitation of cellular metabolism and observation of growth-dependent spectral features bioRxiv 10.1101/2024.01.24.576656 |
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