Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.ejps.2024.106699 |
Lizenz  |
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| Titel (primär) | Pitfalls in evaluating permeability experiments with Caco-2/MDCK cell monolayers |
| Autor | Ebert, A.
; Dahley, C.; Goss, K.-U. |
| Quelle | European Journal of Pharmaceutical Sciences |
| Erscheinungsjahr | 2024 |
| Department | COMPBC |
| Band/Volume | 194 |
| Seite von | art. 106699 |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S0928098724000101-mmc1.pdf https://ars.els-cdn.com/content/image/1-s2.0-S0928098724000101-mmc2.xlsx |
| Keywords | Intrinsic membrane permeability; Caco-2, MDCK; Aqueous boundary layer; Concentration-shift |
| Abstract | When studying the transport of molecules across biological membranes, intrinsic membrane permeability (P0) is more informative than apparent permeability (Papp), because it eliminates external (setup-specific) factors, provides consistency across experiments and mechanistic insight. It is thus an important building block for modeling the total permeability in any given scenario. However, extracting P0 is often difficult, if not impossible, when the membrane is not the dominant transport resistance. In this work, we set out to analyze Papp values measured with Caco-2/MDCK cell monolayers of 69 literature references. We checked the Papp values for a total of 318 different compounds for the extractability of P0, considering possible limitations by aqueous boundary layers, paracellular transport, recovery issues, active transport, a possible proton flux limitation, and sink conditions. Overall, we were able to extract 77 reliable P0 values, which corresponds to about one quarter of the total compounds analyzed, while about half were limited by the diffusion through the aqueous layers. Compared to an existing data set of P0 values published by Avdeef, our approach resulted in a much higher exclusion of compounds. This is a consequence of stricter compound- and reference-specific exclusion criteria, but also because we considered possible concentration-shift effects due to different pH values in the aqueous layers, an effect only recently described in literature. We thus provide a consistent and reliable set of P0, e.g. as a basis for future modeling. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28664 |
| Ebert, A., Dahley, C., Goss, K.-U. (2024): Pitfalls in evaluating permeability experiments with Caco-2/MDCK cell monolayers Eur. J. Pharm. Sci. 194 , art. 106699 10.1016/j.ejps.2024.106699 |
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