Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.jbiotec.2024.01.001
Lizenz creative commons licence
Titel (primär) Glycolate as alternative carbon source for Escherichia coli
Autor Höhmann, S.M.; Briol, T.A.; Ihle, N.; Frick, O.; Schmid, A.; Bühler, B.
Quelle Journal of Biotechnology
Erscheinungsjahr 2024
Band/Volume 381
Seite von 76
Seite bis 85
Sprache englisch
Topic T7 Bioeconomy
Keywords Alternative carbon source; Glycolic acid; Laboratory adaption; Physiology; Glycolate utilization
Abstract The physiology of different Escherichia coli stains was analyzed for growth with glycolate as a potentially promising sustainable sole source of carbon and energy. Different E. coli strains showed large differences regarding lag phases after provision of glycolate. Whereas E. coli W showed fast adaptation, E. coli BW25113, JM101, and BL21 (DE3) needed extensive time for adaption (up to 30 generations) until the attainable µmax was reached, which, at 30 °C, amounted to 0.20–0.25 h−1 for all strains. The overexpression of genes encoding glycolate degradation did neither overcome the need for adaptation of E. coli BL21 (DE3) nor improve growth of E. coli W. Rather, high level expression of proteins involved in uptake and initial degradation steps had an adverse effect on growth. Overall, the results show a promising capacity of E. coli strains for growth on glycolate.
dauerhafte UFZ-Verlinkung
Höhmann, S.M., Briol, T.A., Ihle, N., Frick, O., Schmid, A., Bühler, B. (2024):
Glycolate as alternative carbon source for Escherichia coli
J. Biotechnol. 381 , 76 - 85 10.1016/j.jbiotec.2024.01.001