Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| DOI | 10.1016/j.jchromb.2023.123963 |
| Volltext | Akzeptiertes Manuskript |
| Titel (primär) | Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg |
| Autor | Padioleau, A.; Cariou, R.; Guiffard, I.; Le Bizec, B.; Escher, B.I.
; Antignac, J.-P.; Dervilly, G. |
| Quelle | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences |
| Erscheinungsjahr | 2024 |
| Department | ZELLTOX |
| Band/Volume | 1232 |
| Seite von | art. 123963 |
| Sprache | englisch |
| Topic | T9 Healthy Planet |
| Supplements | https://ars.els-cdn.com/content/image/1-s2.0-S1570023223003732-mmc1.docx https://ars.els-cdn.com/content/image/1-s2.0-S1570023223003732-mmc2.xlsx |
| Keywords | Non-targeted analysis; Clean-up; Lipid removal; High-resolution mass spectrometry; Recoveries; Matrix effects |
| Abstract | Biota samples are used to monitor chemical stressors and their impact on the ecosystem and to describe dietary chemical exposure. These complex matrices require an extraction step followed by clean-up to avoid damaging sensitive analytical instruments based on chromatography coupled to mass spectrometry. While interest for non-targeted analysis (NTA) is increasing, there is no versatile or generic sample preparation for a wide range of contaminants suitable for a diversity of biotic matrices. Among the contaminants’ variety, persistent contaminants are mostly hydrophobic (mid- to non-polar) and bio-magnify through the lipidic fraction. During their extraction, lipids are generally co-extracted, which may cause matrix effect during the analysis such as hindering the acquired signal. The aim of this study was to evaluate the efficacy of four clean-up methods to selectively remove lipids from extracts prior to NTA. We evaluated (i) gel permeation chromatography (GPC), (ii) Captiva EMR-lipid cartridge (EMR), (iii) sulphuric acid degradation (H2SO4) and (iv) polydimethyl siloxane (PDMS) for their efficiency to remove lipids from hen egg extracts. Gas and liquid chromatography coupled with high-resolution mass spectrometry fitted with either electron ionisation or electrospray ionisation sources operating in positive and negative modes were used to determine the performances of the clean-up methods. A set of 102 chemicals with a wide range of physico-chemical properties that covers the chemical space of mid- to non-polar contaminants, was used to assess and compare recoveries and matrix effects. Matrix effects, that could hinder the mass spectrometer signal, were lower for extracts cleaned-up with H2SO4 than for the ones cleaned-up with PDMS, EMR and GPC. The recoveries were satisfactory for both GPC and EMR while those determined for PDMS and H2SO4 were low due to poor partitioning and degradation/dissociation of the compounds, respectively. The choice of the clean-up methods, among those assessed, should be a compromise that takes into account the matrix under consideration, the levels and the physico-chemical properties of the contaminants. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=28470 |
| Padioleau, A., Cariou, R., Guiffard, I., Le Bizec, B., Escher, B.I., Antignac, J.-P., Dervilly, G. (2024): Non-targeted analysis of lipidic extracts by high-resolution mass spectrometry to characterise the chemical exposome: Comparison of four clean-up strategies applied to egg J. Chromatogr. B 1232 , art. 123963 10.1016/j.jchromb.2023.123963 |
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