Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1002/cbic.202300475
Lizenz creative commons licence
Titel (primär) Enlighting electron routes in oxyfunctionalizing Synechocystis sp. PCC 6803
Autor Tüllinghoff, A.; Toepel, J.; Bühler, B.
Quelle Chembiochem
Erscheinungsjahr 2024
Department MIBITECH
Band/Volume 25
Heft 6
Seite von e202300475
Sprache englisch
Topic T7 Bioeconomy
Keywords light-driven biocatalysis; hydroxylation; ferredoxin; photosynthesis; electron transfer
Abstract Phototrophic microorganisms, like cyanobacteria, are gaining attention as host organisms for biocatalytic processes with light as energy source and water as electron source. Redox enzymes, especially oxygenases, can profit from in-situ supply of co-substrates, i.e., reduction equivalents and O2, by the photosynthetic light reaction. The electron transfer downstream of PS I to heterologous electron consuming enzymes in principle can involve NADPH, NADH, and/or ferredoxin, whereas most direct and efficient transfer is desirable. Here, we use the model organism Synechocystis sp. PCC 6803 to investigate, to what extent host and/or heterologous constituents are involved in electron transfer to a heterologous Cytochrome P450 monooxygenase from Acidovorax sp. CHX100. Interestingly, in this highly active light-fueled cycloalkane hydroxylating biocatalyst, host-intrinsic enzymes were found capable of completely substituting the function of the Acidovorax ferredoxin reductase. To a certain extent (20%), this also was true for the Acidovorax ferredoxin. These results indicate the presence of a versatile set of electron carriers in cyanobacteria, enabling efficient and direct coupling of electron consuming reactions to photosynthetic water oxidation. This will both simplify and promote the use of phototrophic microorganisms for sustainable production processes.
dauerhafte UFZ-Verlinkung
Tüllinghoff, A., Toepel, J., Bühler, B. (2024):
Enlighting electron routes in oxyfunctionalizing Synechocystis sp. PCC 6803
ChemBioChem 25 (6), e202300475 10.1002/cbic.202300475