Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.csbj.2023.10.046
Lizenz creative commons licence
Titel (primär) Structure-function and engineering of plant UDP-glycosyltransferase
Autor Wang, M.; Ji, Q.; Lai, B. ORCID logo ; Liu, Y.; Mei, K.
Quelle Computational and Structural Biotechnology Journal
Erscheinungsjahr 2023
Department UMB
Band/Volume 21
Seite von 5358
Seite bis 5371
Sprache englisch
Topic T7 Bioeconomy
Keywords Glycosyltransferase; UGT; Structure; Mechanism; Protein engineering
Abstract Natural products synthesized by plants have substantial industrial and medicinal values and are therefore attracting increasing interest in various related industries. Among the key enzyme families involved in the biosynthesis of natural products, uridine diphosphate-dependent glycosyltransferases (UGTs) play a crucial role in plants. In recent years, significant efforts have been made to elucidate the catalytic mechanisms and substrate recognition of plant UGTs and to improve them for desired functions. In this review, we presented a comprehensive overview of all currently published structures of plant UGTs, along with in-depth analyses of the corresponding catalytic and substrate recognition mechanisms. In addition, we summarized and evaluated the protein engineering strategies applied to improve the catalytic activities of plant UGTs, with a particular focus on high-throughput screening methods. The primary objective of this review is to provide readers with a comprehensive understanding of plant UGTs and to serve as a valuable reference for the latest techniques used to improve their activities.
dauerhafte UFZ-Verlinkung
Wang, M., Ji, Q., Lai, B., Liu, Y., Mei, K. (2023):
Structure-function and engineering of plant UDP-glycosyltransferase
Comp. Struct. Biotechnol. J. 21 , 5358 - 5371 10.1016/j.csbj.2023.10.046