Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.1016/j.biortech.2023.129741
Lizenz creative commons licence
Titel (primär) Production of (hydroxy)benzoate-derived polyketides by engineered Pseudomonas with in situ extraction
Autor Schwanemann, T.; Urban, E.A.; Eberlein, C. ORCID logo ; Gätgens, J.; Rago, D.; Krink, M.; Nikel, P.I.; Heipieper, H.J. ORCID logo ; Wynands, B.; Wierckx, N.
Quelle Bioresource Technology
Erscheinungsjahr 2023
Department UBT
Band/Volume 388
Seite von art. 129741
Sprache englisch
Topic T7 Bioeconomy
Keywords Benzophenones; Polyketide synthesis; Mutasynthesis; Pseudomonas taiwanensis; ISPR by extraction
Abstract Polyketides from (hydroxy)benzoates are an interesting group of plant polyphenolic compounds, whose biotechnological production is so far underrepresented due to their challenging heterologous biosynthesis. Efficient heterologous production of 2,4,6-tri- and 2,3′,4,6-tetrahydroxybenzophenone, 3,5-dihydroxybiphenyl, and 4-hydroxycoumarin by whole-cell biocatalysis in combination with in situ product extraction with an organic solvent was demonstrated. Production was highly dependent on the used CoA ligase and polyketide synthase type III. Therefore, different combinations of polyketide synthases and benzoate-CoA ligases were evaluated for their biosynthesis performance in the solvent-tolerant Pseudomonas taiwanensis VLB120. A solvent screening yielded 2-undecanone as biocompatible, extraction-efficient solvent with good phase separation. In aqueous-organic two-phase cultivations, this solvent extraction circumvents product instability in the aqueous cultivation medium, and it increases yields by reducing inhibitory effects. Complete de novo synthesis from glucose of all (hydroxy)benzoate-derived polyketides was achieved in two-phase cultivations with metabolically engineered strains. Additionally, mutasynthesis was applied to obtain fluorinated benzophenone derivatives.
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Schwanemann, T., Urban, E.A., Eberlein, C., Gätgens, J., Rago, D., Krink, M., Nikel, P.I., Heipieper, H.J., Wynands, B., Wierckx, N. (2023):
Production of (hydroxy)benzoate-derived polyketides by engineered Pseudomonas with in situ extraction
Bioresour. Technol. 388 , art. 129741 10.1016/j.biortech.2023.129741