Details zur Publikation

Referenztyp Zeitschriften
DOI / URL Link
Titel (primär) Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum
Autor Köller, G.; Wichmann, G.; Rolle-Kampczyk, U.; Popp, P.; Herbarth, O.;
Journal / Serie Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Erscheinungsjahr 2006
Department ANA; IMMU; EXPOEPID;
Band/Volume 840
Heft 2
Sprache englisch;
Keywords Capillary electrophoresis; CE-LIF human blood serum; Low sample volume; Ochratoxin A; ELISA
Abstract In this paper the determination of Ochratoxin A (OTA) in low volumes of human blood serum by enzyme-linked immunosorbent assay (ELISA) is compared with an appropriate capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method. In order to use ELISA for high-throughput analysis in epidemiological studies no sample cleanup was performed. Both methods showed a limit of detection (LOD) of 0.5 ng/mL. Comparing the precisions of both methods, the data show that the quantified concentrations in ELISA are higher than the corresponding concentrations in the CE-LIF method. Using a matrix calibration curve instead of a standard calibration curve the reproducibilities of both methods are comparable. No additional matrix effect could be observed by adding phenylalanine as probable matrix compound to the serum.
ID 2793
dauerhafte UFZ-Verlinkung https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=2793
Köller, G., Wichmann, G., Rolle-Kampczyk, U., Popp, P., Herbarth, O. (2006):
Comparison of ELISA and capillary electrophoresis with laser-induced fluorescence detection in the analysis of Ochratoxin A in low volumes of human blood serum
J. Chromatogr. B 840 (2), 94 - 98