Details zur Publikation

Kategorie Textpublikation
Referenztyp Zeitschriften
DOI 10.3390/cancers15174278
Lizenz creative commons licence
Titel (primär) Mast cells retard tumor growth in ovarian cancer: insights from a mouse model
Autor Meyer, N.; Hinz, N.; Schumacher, A.; Weißenborn, C.; Fink, B.; Bauer, M.; von Lenthe, S.; Ignatov, A.; Fest, S.; Zenclussen, A.C.
Quelle Cancers
Erscheinungsjahr 2023
Department IMMU
Band/Volume 15
Heft 17
Seite von art. 4278
Sprache englisch
Topic T9 Healthy Planet
Keywords mast cells; ovarian carcinoma; mouse model; mast cell-deficiency; ultrasonography
Abstract Ovarian cancer has the highest mortality rate among female reproductive tract malignancies. A complex network, including the interaction between tumor and immune cells, regulates the tumor microenvironment, survival, and growth. The role of mast cells (MCs) in ovarian tumor pathophysiology is poorly understood. We aimed to understand the effect of MCs on tumor cell migration and growth using in vitro and in vivo approaches. Wound healing assays using human tumor cell lines (SK-OV-3, OVCAR-3) and human MCs (HMC-1) were conducted. Murine ID8 tumor cells were injected into C57BL6/J wildtype (WT) and MC-deficient C57BL/6-KitW-sh/W-sh (KitW-sh) mice. Reconstitution of KitW-sh was performed by the transfer of WT bone marrow-derived MCs (BMMCs). Tumor development was recorded by high-frequency ultrasonography. In vitro, we observed a diminished migration of human ovarian tumor cells upon direct or indirect MC contact. In vivo, application of ID8 cells into KitW-sh mice resulted in significantly increased tumor growth compared to C57BL6/J mice. Injection of BMMCs into KitW-sh mice reconstituted MCs and restored tumor growth. Our data show that MCs have a suppressive effect on ovarian tumor growth and may serve as a new therapeutic target.
dauerhafte UFZ-Verlinkung
Meyer, N., Hinz, N., Schumacher, A., Weißenborn, C., Fink, B., Bauer, M., von Lenthe, S., Ignatov, A., Fest, S., Zenclussen, A.C. (2023):
Mast cells retard tumor growth in ovarian cancer: insights from a mouse model
Cancers 15 (17), art. 4278 10.3390/cancers15174278