Details zur Publikation |
| Kategorie | Textpublikation |
| Referenztyp | Zeitschriften |
| Titel (primär) | Mixed function oxygenases in cultured fish cells: contributions of in vitro studies to the understanding of MFO induction |
| Autor | Braunbeck, T.; Hauck, C.; Scholz, S.
; Segner, H. |
| Quelle | Zeitschrift für angewandte Zoologie = German journal for applied zoology |
| Erscheinungsjahr | 1996 |
| Department | OEC; COE |
| Band/Volume | 81 |
| Heft | 1 |
| Seite von | 55 |
| Seite bis | 72 |
| Sprache | englisch |
| Keywords | mixed function oxygenase (MFO); cytochrome P-450; fish; cell culture; in vitro; hepatocytes; fibrocytes |
| Abstract | The expression of toxicological responses in biological
systems can be studied by various approaches and methodologies. In comparison
to in vivo approaches, in vitro systems provide several distinct advantages (BAKSI
& FRAZIER 1990, BRAUNBECK 1993, BRAUNBECK & STORCH 1992, SEGNER &
LENZ 1993, ZAHN et aL 1993): (1) Technical reasons. Improved standardization of experimental conditions allows for better control of influences on the test systems by biological, chemical an physical factors and, thus, decreases variability of results; repeated sampling over time within the same preparation is possible; miniaturization of assay volumes drastically reduces health risks for personnel and amounts of hazardous wastes in toxicological experiments; requirements for space and equipment are usually reduced; in most cases, in vitro tests are quicker and cheaper to perform than in vivo studies. (2) Ethical considerations. The number of animals spent for toxicological experiments can be significantly lowered. (3) Scientific reasons. Elimination of systemic effects allows for the analysis of molecular and cellular mechanisms of toxic action; since biological variation is greatly reduced in cell systems, they provide a good experimental basis to study the effects of chemical variation, i. e. to establish structure-activity relationships. |
| dauerhafte UFZ-Verlinkung | https://www.ufz.de/index.php?en=20939&ufzPublicationIdentifier=27618 |
| Braunbeck, T., Hauck, C., Scholz, S., Segner, H. (1996): Mixed function oxygenases in cultured fish cells: contributions of in vitro studies to the understanding of MFO induction Z. Angew. Zool. 81 (1), 55 - 72 |
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