Details zur Publikation
Kategorie Textpublikation
Referenztyp Zeitschriften
Titel (primär) Microbiological aspects of methane emission by a ricefield soil from the Camargue (France): 1. Methanogenesis and related microflora
Autor Joulian, C.; Ollivier, B.; Neue, H.-U.; Roger, P.A.
Quelle European Journal of Soil Biology
Erscheinungsjahr 1996
Department BOFO; BOSYS
Band/Volume 32
Heft 2
Seite von 61
Seite bis 70
Sprache englisch
Keywords Methane; rice; soil; acetotrophs; hydrogenotrophs; methylotrophs; formatotrophs; cellulose; xylan; desiccation; straw; algae
Abstract The major trophic groups of methanogens and the methanogenic potential of a rice soil from Camargue
(France) were studied under laboratoiy conditions, with regard to the effects of soil desiccation and
the addition of straw or algal material. The dynamics of cellulose and xylan fermentation by the soil
microflora were also established.
Methanogens remained viable for several months in dry oxic soil, indicating that their density at
the end of the crop cycle might not be markedly reduced by the dry fallow that usually follows
rice harvest. Methanogenic activities were maximum two weeks after soil submersion. The native soil
organic matter mineralized after submersion favoured acetotrophic methanogens, while adding straw
or algae favoured hydrogenotrophic methanogens. Straw or algae addition increased populations of
hydrogenotrophs and formatotrophs similarly, while four times more CH4 was produced with straw than
with algae. Methanogenesis appeared to be more limited by the availability and nature of the substrate
than by the density of methanogens. Cellulose fermentation by the soil microflora exhibited a first stage,
where hydrogenotrophs were mostly responsible for methanogenesis through interspecies H2 transfer, and
a second stage, where acetotrophs consumed accumulated acetate. Counts, microscopic observations, and
strain isolations showed a diversified methanogenic microflora including rods, sarcinae, cocci and spirilla.
Four mesophilic, neutrophilic strains were isolated and phenotypically characterized.
Joulian, C., Ollivier, B., Neue, H.-U., Roger, P.A. (1996):
Microbiological aspects of methane emission by a ricefield soil from the Camargue (France): 1. Methanogenesis and related microflora
Eur. J. Soil Biol. 32 (2), 61 - 70